Dataset Information

Assessment of contrast perception of obstacles in a tunnel entrance.

ABSTRACT: Background: The purpose of this study was to assess the contrast perception of obstacles in a tunnel entrance which was placed in Ilam Province, Iran. Methods: An obstacle with the reflection coefficient of 20% was mounted at the entrance of thetunnel and then, the car was placed near the tunnel entrance and the intrinsic luminance of the road (L_r,intrinsic) and obstacle (L_o,intrinsic) were measured inside the car using the luminance meter. Results: Intrinsic luminance of obstacle and road at the entrance of the tunnel were measured as 41 and 17 cd/m², respectively. The contrast perception of obstacle in deriver's eyes and in the safe stopping distance (SSD) from the tunnel entrance was determined as 2.79 cd/m². At the entrance of the studied tunnel, the contrast perception of obstacles with the reflection coefficient of 20% was lower than the minimum contrast perception (28%) recommended by International Commission on Illumination. Conclusion: The main conclusion that can be drawn from it, is that this obstacle in the SSD from the tunnel entrance cannot be conceived by the drivers, which may lead to higher rate of road traffic crashes.

SUBMITTER: Mehri A

PROVIDER: S-EPMC6249494 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Assessment of contrast perception of obstacles in a tunnel entrance.

Mehri Ahmad A Farhang Dehghan Somayeh S Abbasi Milad M Beheshti Mohammad Hosein MH Sajedifar Javad J Jafari Sayed Mohammad SM Khadem Monireh M Hajizadeh Roohalah R

Health promotion perspectives 20181027 4

Background: The purpose of this study was to assess the contrast perception of obstacles in a tunnel entrance which was placed in Ilam Province, Iran. Methods: An obstacle with the reflection coefficient of 20% was mounted at the entrance of thetunnel and then, the car was placed near the tunnel entrance and the intrinsic luminance of the road (Lr,intrinsic) and obstacle (Lo,intrinsic) were measured inside the car using the luminance meter. Results: Int ...[more]

PMID: 30479980

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Project description:Handling of laboratory mice is essential for experiments and husbandry, but handling can increase anxiety in mice, compromising their welfare and potentially reducing replicability between studies. The use of non-aversive handling (e.g., tunnel handling or cupping), rather than the standard method of picking mice up by the tail, has been shown to enhance interaction with a handler, reduce anxiety-like behaviours, and increase exploration and performance in standard behavioural tests. Despite this, some labs continue to use tail handling for routine husbandry, and the extent to which non-aversive methods are being used is currently unknown. Here we conducted an international online survey targeting individuals that work with and/or conduct research using laboratory mice. The survey aimed to identify the handling methods currently being used, and to determine common obstacles that may be preventing the wider uptake of non-aversive handling. We also surveyed opinions concerning the current data in support of non-aversive handling for mouse welfare and scientific outcomes. 390 complete responses were received and analysed quantitatively and thematically. We found that 35% report using tail handling only, and 43% use a combination of tail and non-aversive methods. 18% of respondents reported exclusively using non-aversive methods. The vast majority of participants were convinced that non-aversive handling improves animal welfare and scientific outcomes. However, the survey indicated that researchers were significantly less likely to have heard of non-aversive handling and more likely to use tail handling compared with animal care staff. Thematic analysis revealed there were concerns regarding the time required for non-aversive methods compared with tail handling, and that there was a perceived incompatibility of tunnel handling with restraint, health checks and other routine procedures. Respondents also highlighted a need for additional research into the impact of handling method that is representative of experimental protocols and physiological indicators used in the biomedical fields. This survey highlights where targeted research, outreach, training and funding may have the greatest impact on increasing uptake of non-aversive handling methods for laboratory mice.

Project description:BACKGROUND: Mutation of Trp-40 in the Cel7A cellobiohydrolase from Trichoderma reesei (TrCel7A) causes a loss of crystalline cellulose-degrading ability. RESULTS: Mutant W40A showed reduced specific activity for crystalline cellulose and diffused the cellulose chain from the entrance of the active site tunnel. CONCLUSION: Trp-40 is essential for chain end loading to initiate processive hydrolysis of TrCel7A. SIGNIFICANCE: The mechanisms of crystalline polysaccharide degradation are clarified. The glycoside hydrolase family 7 cellobiohydrolase Cel7A from Trichoderma reesei is one of the best studied cellulases with the ability to degrade highly crystalline cellulose. The catalytic domain and the cellulose-binding domain (CBD) are both necessary for full activity on crystalline substrates. Our previous high-speed atomic force microscopy studies showed that mutation of Trp-40 at the entrance of the catalytic tunnel drastically decreases the ability to degrade crystalline cellulose. Here, we examined the activities of the WT enzyme and mutant W40A (with and without the CBD) for various substrates. Evaluation and comparison of the specific activities of the enzymes (WT, W40A, and the corresponding catalytic subunits (WTcat and W40Acat)) adsorbed on crystalline cellulose indicated that Trp-40 is involved in recruiting individual substrate chains into the active site tunnel to initiate processive hydrolysis. This was supported by molecular dynamics simulation study, i.e. the reducing end glucose unit was effectively loaded into the active site of WTcat, but not into that of W40Acat, when the simulation was started from subsite -7. However, when similar simulations were carried out starting from subsite -5, both enzymes held the substrate for 50 ns, indicating that the major difference between WTcat and W40Acat is the length of the free chain end of the substrate required to allow initiation of processive movements; this also reflects the difference between crystalline and amorphous celluloses. The CBD is important for enhancing the enzyme population on crystalline substrate, but it also decreases the specific activity of the adsorbed enzyme, possibly by attaching the enzyme to non-optimal places on the cellulose surface and/or hindering processive hydrolysis.

Dataset Information

Assessment of contrast perception of obstacles in a tunnel entrance.

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Assessment of contrast perception of obstacles in a tunnel entrance.

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