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A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells.

ABSTRACT: We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (?52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23?nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.

SUBMITTER: Ma X

PROVIDER: S-EPMC6311829 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells.

Ma Xiaohua X Wu Guoguang G Zhao Yuehua Y Yuan Zibo Z Zhang Yu Y Xia Ning N Yang Mengnan M Liu Lin L

Journal of analytical methods in chemistry 20181213

We reported here a turn-on fluorescent probe (<b>1</b>) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe <b>1</b> also displays high selectivity toward ...[more]

PMID: 30647984

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