Ontology highlight
ABSTRACT:
SUBMITTER: Thompson AJ
PROVIDER: S-EPMC6333438 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Thompson Amelia J AJ Pillai Eva K EK Dimov Ivan B IB Foster Sarah K SK Holt Christine E CE Franze Kristian K
eLife 20190115
Tissue mechanics is important for development; however, the spatio-temporal dynamics of in vivo tissue stiffness is still poorly understood. We here developed tiv-AFM, combining time-lapse in vivo atomic force microscopy with upright fluorescence imaging of embryonic tissue, to show that during development local tissue stiffness changes significantly within tens of minutes. Within this time frame, a stiffness gradient arose in the developing <i>Xenopus</i> brain, and retinal ganglion cell axons ...[more]