Ontology highlight
ABSTRACT:
SUBMITTER: Webster MW
PROVIDER: S-EPMC6340701 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Webster Michael W MW Stowell James Aw JA Passmore Lori A LA
eLife 20190102
The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. Here, we reconstitute accelerated and selective deadenylation of RNAs containing AU-rich elements (AREs) and Pumilio-response elements (PREs). We find that the fission yeast homologues of Tristetraprolin/TTP and Pumilio/Puf (Zfs1 and Puf3) interact with Ccr4-Not v ...[more]