Ontology highlight
ABSTRACT:
SUBMITTER: Harada K
PROVIDER: S-EPMC6405243 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Harada Kumi K Kotani Tetsuya T Kirisako Hiromi H Sakoh-Nakatogawa Machiko M Oikawa Yu Y Kimura Yayoi Y Hirano Hisashi H Yamamoto Hayashi H Ohsumi Yoshinori Y Nakatogawa Hitoshi H
eLife 20190227
In autophagy, Atg proteins organize the pre-autophagosomal structure (PAS) to initiate autophagosome formation. Previous studies in yeast revealed that the autophagy-related E3 complex Atg12-Atg5-Atg16 is recruited to the PAS via Atg16 interaction with Atg21, which binds phosphatidylinositol 3-phosphate (PI3P) produced at the PAS, to stimulate conjugation of the ubiquitin-like protein Atg8 to phosphatidylethanolamine. Here, we discover a novel mechanism for the PAS targeting of Atg12-Atg5-Atg16, ...[more]