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Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis.

ABSTRACT: Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins (C/D snoRNPs) is guided by conserved trans-acting factors that act collectively to assemble the core proteins SNU13/Snu13, NOP58/Nop58, NOP56/Nop56, FBL/Nop1, and box C/D small nucleolar RNAs (C/D snoRNAs), in human and in yeast, respectively. This finely elaborated process involves the sequential interplay of snoRNP-related proteins and RNA through the formation of transient pre-RNP complexes. BCD1/Bcd1 protein is essential for yeast cell growth and for the specific accumulation of box C/D snoRNAs. In this work, chromatin, RNA, and protein immunoprecipitation assays revealed the ordered loading of several snoRNP-related proteins on immature and mature snoRNA species. Our results identify Bcd1p as an assembly factor of C/D snoRNP biogenesis that is likely recruited cotranscriptionally and that directs the loading of the core protein Nop58 on RNA.

SUBMITTER: Paul A

PROVIDER: S-EPMC6426285 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis.

Paul Arnaud A Tiotiu Decebal D Bragantini Benoît B Marty Hélène H Charpentier Bruno B Massenet Séverine S Labialle Stéphane S

RNA (New York, N.Y.) 20190130 4

Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins (C/D snoRNPs) is guided by conserved <i>trans</i>-acting factors that act collectively to assemble the core proteins SNU13/Snu13, NOP58/Nop58, NOP56/Nop56, FBL/Nop1, and box C/D small nucleolar RNAs (C/D snoRNAs), in human and in yeast, respectively. This finely elaborated process involves the sequential interplay of snoRNP-related proteins and RNA through the formation of transient pre-RNP complexes. BCD1/Bcd1 protein is essent ...[more]

PMID: 30700579

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