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Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination.


ABSTRACT: Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell culture growth on metal films is not as universally available as growth on dielectric substrates. Here we show that use of azimuthal rotation illumination allows SPRM spatial resolution to be enhanced by up to an order of magnitude. The metal film can also be replaced by a dielectric multilayer and then a different label-free surface-sensitive photonic microscopy is developed, which has more choices in terms of the working wavelength, polarization, and imaging section, and will bring opportunities for applications in biology.

SUBMITTER: Kuai Y 

PROVIDER: S-EPMC6440756 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination.

Kuai Yan Y   Chen Junxue J   Tang Xi X   Xiang Yifeng Y   Lu Fengya F   Kuang Cuifang C   Xu Liang L   Shen Weidong W   Cheng Junjie J   Gui Huaqiao H   Zou Gang G   Wang Pei P   Ming Hai H   Liu Jianguo J   Liu Xu X   Lakowicz Joseph R JR   Zhang Douguo D  

Science advances 20190329 3


Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell cultu  ...[more]

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