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Taxonomic characterization and antimicrobial compound production from Streptomyces chumphonensis BDK01 isolated from marine sediment.

ABSTRACT: Streptomyces sp. isolated from marine sediment collected from Palk Strait, Bay of Bengal was investigated for its antagonistic potential. The isolate exhibited antimicrobial activity against selected bacterial strains of clinical importance such as Staphylococcus aureus MTCC 3160, Bacillus pumilus NCIM 2327, S. aureus (methicillin resistant), Escherichia coli MTCC 1698, E. coli (ESBL), Shigella flexneri MTCC 1457, Proteus vulgaris and Enterobacter cloacae. Phenotypic and molecular characterization ascertained the isolate BDK01 as Streptomyces chumphonensis. Media optimization with one variable-at-a-time strategy was attempted to identify the ideal concentrations of starch (5-15 g/l), casein (0.01-0.05 g/l), NaCl 1.0-3.0 g/l, pH (4.0-9.0 g/l), temperature (25-45 °C) and inoculum level (0.5-5 ml) towards achieving maximum antimicrobial compound production. Statistical optimization of production media was carried by establishing an 11 variables 17 run experiment through PB model which evinced starch, calcium carbonate, pH and inoculum concentration that highly influenced bioactive compound production. Spectral data of active ethyl acetate extract revealed the presence of various bioactive compounds such as Salicyl alcohol, N-phenyl benzamide. 6-Octadecenoic acid, (Z), 1,3,5-Cycloheptatriene. Antiproliferation activity of active fraction against MCF-7 Cell line exhibited IC₅₀ value of 9.5 µg/ml. Overall, it is observed that the marine actinomycete S. chumphonensis BDK01 could be employable as promising strain for novel antimicrobial and cytotoxic metabolites.

SUBMITTER: Manikandan M

PROVIDER: S-EPMC6449414 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Taxonomic characterization and antimicrobial compound production from Streptomyces chumphonensis BDK01 isolated from marine sediment.

Manikandan Madheslu M Gowdaman Vasudevan V Duraimurugan Kasiviswanathan K Prabagaran Solai Ramatchandirane SR

3 Biotech 20190404 5

Streptomyces sp. isolated from marine sediment collected from Palk Strait, Bay of Bengal was investigated for its antagonistic potential. The isolate exhibited antimicrobial activity against selected bacterial strains of clinical importance such as Staphylococcus aureus MTCC 3160, Bacillus pumilus NCIM 2327, S. aureus (methicillin resistant), Escherichia coli MTCC 1698, E. coli (ESBL), Shigella flexneri MTCC 1457, Proteus vulgaris and En ...[more]

PMID: 30997304

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Project description:ObjectiveTo investigate the cytotoxic activity of actinomycete isolated from marine sediment.MethodsIn the present study the DNA was isolated and the ITS region of 16s rRNA was amplified by polymerase chain reaction, using two universal bacterial primers, 1492R (5'-GGTTACCTTGTTAC GACTT-3') and Eubac27F (5'-AGAGTTTGATCCTGGCTC AG-3'). The amplified products were purified using TIANgel mini purification kit, ligated to MD18-T simple vector (TaKaRa), and transformed into competent cells of Escherichia coli DH5α. 16S rRNA gene fragment was sequenced using forward primer M13F (-47) and reverse primer M13R (-48). Blast search sequence similarity was found against the existing non-redundant nucleotide sequence database thus, identified as Streptomyces sp SU, Streptomyces rubralavandulae strain SU1, Streptomyces cacaoi strain SU2, Streptomyces cavourensis strain SU3, Streptomyces avidinii strain SU4, Streptomyces globisporus strain SU5, Streptomyces variabilis strain SU6, Streptomyces coelicolor strain SU 7. Among the eight identified isolates, one actinomycete Streptomyces avidinii strain SU4 was selected for further study.ResultsCrude extract of the actinomycete isolate exhibited IC50 in 64.5 µg against Hep-2 cell line, 250 µg in VERO cell line. This value is very close to the criteria of cytotoxicity activity for the crude extracts, as established by the American National Cancer Institute (NCI) is in IC50 < 30 µg/mL. The GC MS analysis showed that the active principle might be 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester (12.17%), isooctyl phthalate (15.29%) with the retention time 15.642 and 21.612, respectively.ConclusionsThis study clearly proves that the marine sediment derived actinomycetes with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical and anticancer screening programs. These results help us to conclude that the potential of using metabolic engineering and post genomic approaches to isolate more bioactive compounds and make their possible commercial application is not far off.

Dataset Information

Taxonomic characterization and antimicrobial compound production from Streptomyces chumphonensis BDK01 isolated from marine sediment.

Publications

Taxonomic characterization and antimicrobial compound production from <i>Streptomyces chumphonensis</i> BDK01 isolated from marine sediment.

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