Ontology highlight
ABSTRACT:
SUBMITTER: Zhu H
PROVIDER: S-EPMC6450418 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Zhu Haibao H Zhang Linlin L Tong Sheng S Lee Ciaran M CM Deshmukh Harshavardhan H Bao Gang G
Nature biomedical engineering 20181112 2
The potential of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9)-based therapeutic genome editing is hampered by difficulties in the control of the in vivo activity of CRISPR-Cas9. To minimize any genotoxicity, precise activation of CRISPR-Cas9 in the target tissue is desirable. Here, we show that, by complexing magnetic nanoparticles with recombinant baculoviral vectors (MNP-BVs), CRISPR-Cas9-mediated genome editing can be activated locally ...[more]