Ontology highlight
ABSTRACT:
SUBMITTER: Tran NT
PROVIDER: S-EPMC6503098 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Tran Ngoc-Tung NT Bashir Sanum S Li Xun X Rossius Jana J Chu Van Trung VT Rajewsky Klaus K Kühn Ralf R
Frontiers in genetics 20190430
The CRISPR-Cas9 system is used for genome editing in mammalian cells by introducing double-strand breaks (DSBs) which are predominantly repaired via non-homologous end joining (NHEJ) or to lesser extent by homology-directed repair (HDR). To enhance HDR for improving the introduction of precise genetic modifications, we tested fusion proteins of Cas9 nuclease with HDR effectors to enforce their localization at DSBs. Using a traffic-light DSB repair reporter (TLR) system for the quantitative detec ...[more]