Ontology highlight
ABSTRACT:
SUBMITTER: Pabis M
PROVIDER: S-EPMC6511859 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Pabis Marta M Corsini Lorenzo L Vincendeau Michelle M Tripsianes Konstantinos K Gibson Toby J TJ Brack-Werner Ruth R Sattler Michael M
Nucleic acids research 20190501 9
The HIV-1 protein Rev is essential for virus replication and ensures the expression of partially spliced and unspliced transcripts. We identified a ULM (UHM ligand motif) motif in the Arginine-Rich Motif (ARM) of the Rev protein. ULMs (UHM ligand motif) mediate protein interactions during spliceosome assembly by binding to UHM (U2AF homology motifs) domains. Using NMR, biophysical methods and crystallography we show that the Rev ULM binds to the UHMs of U2AF65 and SPF45. The highly conserved Trp ...[more]