Dtar-Finder: program for drug target identification and characterization in bacteria.

ABSTRACT: The drug target identification is the primary step for drug discovery. Recent development of computational techniques and availability of sequencing data has provided numerous opportunities for target identification but very few of them are fully automated. Here, we have developed a Perl program named Dtar-Finder for drug target identification and its characterization. Dtar-Finder predicts the drug targets which are essential to pathogen and non homologous to human, essential human anti-targets and gut microflora. This program is divided in 6 modules where modules 1-4 extract drug targets while module 5-6 predicts druggability and broad spectrum ability of identified candidates. The performance of this program in terms of sensitivity and specificity is calculated where specificity score was better compare to sensitivity score. Further, we have tested our script on C. botulinum (3572 proteins) and 35 potential drug targets have been identified. Out of which 16 broad spectrums candidates were predicted whereas 8 candidates are found to be druggable whiles remaining are considered to be 'novel'. These drug targets were cross-validated through literature showing 77.14% accuracy. Thus, the idea behind this work was to develop a fast, robust and generic program capable of finding drug targets in bacteria, which has been fulfilled satisfactorily.

SUBMITTER: Prasad P 

PROVIDER: S-EPMC6637396 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature