Dataset Information

Fabrication of an anatomy-mimicking BIO-AIR-TUBE with engineered cartilage.

ABSTRACT:

Introduction

We devised a strategy for the fabrication of an 'anatomy-mimicking' cylinder-type engineered trachea combined with cartilage engineering. The engineered BIOTUBEs are used to support the architecture of the body tissue, for long-segment trachea (>5 cm) with carinal reconstruction. The aim of the present study was to fabricate an anatomy-mimicking cylinder-type regenerative airway, and investigate its applicability in a rabbit model.

Methods

Collagen sponge rings (diameter: 6 mm) were arranged on a silicon tube (diameter: 6 mm) at 2-mm intervals. Chondrocytes from the auricular cartilage were seeded onto collagen sponges immediately prior to implantation in an autologous manner. These constructs were embedded in dorsal subcutaneous pouches of rabbits. One month after implantation, the constructs were retrieved for histological examination. In addition, cervical tracheal sleeve resection was performed, and these engineered constructs were implanted into defective airways through end-to-end anastomosis.

Results

One month after implantation, the engineered constructs exhibited similar rigidity and flexibility to those observed with the native trachea. Through histological examination, the constructs showed an anatomy-mimicking tracheal architecture. In addition, the engineered constructs could be anastomosed to the native trachea without air leakage.

Conclusion

The present study provides the possibility of generating anatomy-mimicking cylinder-type airways, termed BIO-AIR-TUBEs, that engineer cartilage in an in-vivo culture system. This approach involves the use of BIOTUBEs formed via in-body tissue architecture technology. Therefore, the BIO-AIR-TUBE may be useful as the basic architecture of artificial airways.

SUBMITTER: Komura M

PROVIDER: S-EPMC6700413 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Publications

Fabrication of an anatomy-mimicking BIO-AIR-TUBE with engineered cartilage.

Komura Makoto M Komura Hiroko H Satake Ryosuke R Suzuki Keisuke K Yonekawa Hironobu H Ikebukuro Kenichi K Komuro Hiroaki H Hoshi Kazuto K Takato Tsuyoshi T Moriwaki Takeshi T Nakayama Yasuhide Y

Regenerative therapy 20190808

<h4>Introduction</h4>We devised a strategy for the fabrication of an 'anatomy-mimicking' cylinder-type engineered trachea combined with cartilage engineering. The engineered BIOTUBEs are used to support the architecture of the body tissue, for long-segment trachea (>5 cm) with carinal reconstruction. The aim of the present study was to fabricate an anatomy-mimicking cylinder-type regenerative airway, and investigate its applicability in a rabbit model.<h4>Methods</h4>Collagen sponge rings (diame ...[more]

PMID: 31453272

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Dataset Information

Fabrication of an anatomy-mimicking BIO-AIR-TUBE with engineered cartilage.

Introduction

Methods

Results

Conclusion

Publications

Fabrication of an anatomy-mimicking BIO-AIR-TUBE with engineered cartilage.

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