Ontology highlight
ABSTRACT:
SUBMITTER: Schussler S
PROVIDER: S-EPMC6718149 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Schüssler Sonja S Haase Ilka I Perbandt Markus M Illarionov Boris B Siemens Alexandra A Richter Klaus K Bacher Adelbert A Fischer Markus M Gräwert Tobias T
Acta crystallographica. Section F, Structural biology communications 20190830 Pt 9
A putative open reading frame encoding GTP cyclohydrolase I from Listeria monocytogenes was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified and was confirmed to convert GTP to dihydroneopterin triphosphate (K<sub>m</sub> = 53 µM; v<sub>max</sub> = 180 nmol mg<sup>-1</sup> min<sup>-1</sup>). The protein was crystallized from 1.3 M sodium citrate pH 7.3 and the crystal structure was solved at a resolution of 2.4 Å (R<sub>free</sub> = 0.226) by molecular rep ...[more]