Ontology highlight
ABSTRACT:
SUBMITTER: Thirion L
PROVIDER: S-EPMC6722894 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Thirion Laurence L Pezzi Laura L Corcostegui Iban I Dubot-Pérès Audrey A Falchi Alessandra A de Lamballerie Xavier X Charrel Remi N RN
Viruses 20190815 8
Chikungunya virus (CHIKV) re-emerged as a globalized health threat fifteen years ago. There are dozens of RT-PCR assays published. An inventory of the latter was made, and after <i>in silico</i> analysis, two assays were selected for their ability to detect strains belonging to the five CHIKV genetic lineages. They were combined in order to provide a robust assay not affected by genetic point mutations and the resulting Duo CHIKV real-time RT-PCR assay was compared to the two parental single-ple ...[more]