Ontology highlight
ABSTRACT:
SUBMITTER: Whedon SD
PROVIDER: S-EPMC6784325 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Whedon Samuel D SD Parker Marissa K MK Tyson Elizabeth L EL Ritterhoff Tobias T Shelton Patrick M M PMM Chatterjee Champak C
Chemical communications (Cambridge, England) 20190201 14
The Cu(i)-mediated click reaction of proteins with affinity tags enables their selective isolation from complex mixtures. However, irreversible protein modification limits the interpretation of results from subsequent biophysical and biochemical assays. We report a facile and modular chemical strategy to reversibly modify peptides and proteins with biotin and FLAG affinity tags at a clickable glutamine (CliQ) residue. ...[more]