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A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO2 Derivatives and Its Applications in Bioimaging.

ABSTRACT: Based upon the intramolecular charge transfer (ICT) mechanism, a novel ratiometric fluorescent probe EB was developed to detect SO32-/HSO3-. The probe displayed both colorimetric and ratiometric responses toward SO32-/HSO3-. It displayed a quick response (within 60 s), good selectivity and high sensitivity (a detection limit of 28 nM) towards SO32-/HSO3-. The SO32-/HSO3- sensing mechanism was confirmed as the Michael addition reaction by ESI-MS. Moreover, the probe could be applied to measure the level of sulfite in real samples, like sugar and chrysanthemum, and it could also be used to detect SO32-/HSO3- in HepG2 cells through confocal fluorescence microscopy, which proved its practical application in clinical diagnosis.

SUBMITTER: Qin YH

PROVIDER: S-EPMC6865185 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO2 Derivatives and Its Applications in Bioimaging.

Qin Yan-Hong YH Jiang Xiao-Yi XY Que Yuan-Fang YF Gu Jing-Yi JY Wu Tong T Aihemaiti Ayinazhaer A Shi Ke-Xin KX Kang Wen-Yu WY Hu Bi-Ying BY Lan Jin-Shuai JS Ding Yue Y Zhang Tong T

Molecules (Basel, Switzerland) 20191105 21

Based upon the intramolecular charge transfer (ICT) mechanism, a novel ratiometric fluorescent probe EB was developed to detect SO32-/HSO3-. The probe displayed both colorimetric and ratiometric responses toward SO32-/HSO3-. It displayed a quick response (within 60 s), good selectivity and high sensitivity (a detection limit of 28 nM) towards SO32-/HSO3-. The ...[more]

PMID: 31694349

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