Dataset Information

A single nucleotide polymorphism of the interferon-? gene and susceptibility to hepatitis B virus-related cirrhosis: a randomized controlled trial.

ABSTRACT: OBJECTIVE:This study aimed to explore the correlation between a single nucleotide polymorphism (SNP) of the interferon-? (IFN-?) gene and susceptibility to hepatitis B virus (HBV) -related cirrhosis in the Chinese population. METHOD:PCR-LDR was employed for the genotyping of two SNPs, rs1861494 and rs2069718, of the IFN-? gene in 230 patients with HBV-related cirrhosis and 320 inactive HBsAg carriers without cirrhosis. It was then determined whether the Hardy-Weinberg (H-W) equilibrium was satisfied. The odds ratio (OR) and 95% confidence interval (CI) were analyzed using the chi-square test and univariate non-conditional logistic regression. Haplotypes were established using SHEsis and SNPStats online software and their interaction with non-genetic factors was analyzed. RESULTS:Compared with the AA genotype of rs1861494 SNP, AG+GG genotypes increased the risk of HBV-related cirrhosis. There was a significant difference in the distribution of A and G alleles between the case group and the control group (P<0.05). There was also a significant difference in the distribution of AA, AG, GG, AA, and AG+GG genotypes and A/G alleles between the case group and the control group (P<0.05). This indicated that the G allele may be a risk factor for HBV-related cirrhosis. By analyzing the different distribution of haplotypes in the case group and the control group, we observed significant differences in the distribution of AA, AG and GA haplotypes between the case and control groups (P<0.05). Haplotypes of the IFN-? gene did not interact with other relevant factors. CONCLUSION:The G allele of rs1861494 SNP as well as AG and GG genotypes and G allele of rs2069718 SNP may be risk factors of HBV-related cirrhosis. The AA haplotype may be a protective factor for HBV-related cirrhosis, while the AG haplotype is a risk factor for HBV-related cirrhosis.

SUBMITTER: Wang Q

PROVIDER: S-EPMC6962819 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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A single nucleotide polymorphism of the interferon-γ gene and susceptibility to hepatitis B virus-related cirrhosis: a randomized controlled trial.

Wang Qing Q Chang Fangzhi F Li Wei W

International journal of clinical and experimental pathology 20180801 8

<h4>Objective</h4>This study aimed to explore the correlation between a single nucleotide polymorphism (SNP) of the interferon-γ (IFN-γ) gene and susceptibility to hepatitis B virus (HBV) -related cirrhosis in the Chinese population.<h4>Method</h4>PCR-LDR was employed for the genotyping of two SNPs, rs1861494 and rs2069718, of the IFN-γ gene in 230 patients with HBV-related cirrhosis and 320 inactive HBsAg carriers without cirrhosis. It was then determined whether the Hardy-Weinberg (H-W) equili ...[more]

PMID: 31949806

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Project description:BackgroundGenetic polymorphisms in the RAS gene family are associated with different diseases, which may include alcohol-related disorders. Previous studies showed an association of the allelic variant rs26907 in RASGRF2 gene with higher alcohol intake. Additionally, the rs61764370 polymorphism in the KRAS gene is located in a binding site for the let-7 micro-RNA family, which is potentially involved in alcohol-induced inflammation. Therefore, this study was designed to explore the association between these two polymorphisms and susceptibility to alcoholism or alcoholic liver disease (ALD).MethodsWe enrolled 301 male alcoholic patients and 156 healthy male volunteers in this study. Polymorphisms were genotyped by using TaqMan® PCR assays for allelic discrimination. Allelic and genotypic frequencies were compared between the two groups. Logistic regression analysis was performed to analyze the inheritance model.ResultsThe A allele of the RASGRF2 polymorphism (rs26907) was significantly more prevalent among alcoholic patients with cirrhosis (23.2%) compared to alcoholic patients without ALD (14.2%). This difference remained significant in the group of patients with alcohol dependence (28.8% vs. 14.3%) but not in those with alcohol abuse (15.1% vs. 14.4%). Multivariable logistic regression analysis showed that the A allele of this polymorphism (AA or GA genotype) was associated with alcoholic cirrhosis both in the total group of alcoholics (odds ratio [OR]: 2.33, 95% confidence interval [CI]: 1.32-4.11; P = 0.002) and in the group of patients with alcohol dependence (OR: 3.1, 95% CI: 1.50-6.20; P = 0.001). Allelic distributions of the KRAS polymorphism (rs61764370) did not differ between the groups.ConclusionsTo our knowledge, this genetic association study represents the first to show an association of the RASGRF2 G>A (rs26907) polymorphism with ALD in men, particularly in the subgroup of patients with AD. The findings suggest the potential relevance of the RAS gene family in alcoholism and ALD.

Project description:BackgroundGynecological malignant tumor is a serious threat to women's health, cervical cancer, endometrial cancer and ovarian cancer are the most common. The eponymous protein encoded by the XRCC1 (X-ray repair cross complementation 1) gene is an important functional protein in the process of single-stranded DNA damage. Non-synonymous mutations of XRCC1 gene cause amino acid sequence changes that affect protein function and DNA repair ability, and may affect the interaction with other DNA repair proteins, leading to increased risk of tumor development. Many studies have assessed the association between XRCC1 gene polymorphism and the risk of cancer in the female reproductive system, but the results have been inconclusive. In this study, the relationship between XRCC1 Arg399Gln, Arg194Trp, Arg280His single nucleotide polymorphisms and susceptibility to gynecological malignancies was further explored by meta-analysis.MethodsEnglish database: Pubmed, Medline, Excerpta Medica Database, Cochrance, etc; Chinese database: China national knowledge infrastructure, Wanfang Database, etc. STATA14 was used for statistical analysis, such as odd ratio (OR) value, subgroup analysis, heterogeneity test, sensitivity analysis, and publication bias.ResultsIn gynecologic cancers, the allele frequency difference of Arg399Gln case control group was statistically significant (GvsA: P = .007). There was no significant difference in allele frequency in the Arg194Trp and Arg280His case control groups (P = .065, 0.198). In different gene models, Arg399Gln was significantly correlated with gynecologic cancers susceptibility (GGvs AA: OR 0.91; 95% confidence interval [CI], 0.85 0.98); Arg194Trp was significantly correlated with gynecologic cancers susceptibility (CCvs TT: OR 0.94; 95% CI 0.88,1.00; CCvs CT: OR 0.97; 95% CI 0.90, 1.05); Arg280His was significantly correlated with gynecologic cancers susceptibility (GGvs AA: OR 0.98; 95% CI 0.94, 1.02; GGvs GA: OR 1.00;95% CI 0.97, 1.04). In the subgroup analysis, Arg399Gln and Arg194Trp were significantly correlated with gynecologic cancers susceptibility in the Asian race (P = .000, 0.049). In the analysis of different cancer subgroups, Arg399Gln and cervical cancer susceptibility were statistically significant (P = .039). Arg194Trp and endometrial cancer susceptibility were statistically significant (P = .033, 0.001).ConclusionsXRCC1 Arg399Gln, Arg194Trp, Arg280His single nucleotide polymorphisms were associated with gynecologic cancer susceptibility. Arg399Gln genotype was statistically significant in relation to cervical cancer susceptibility. Arg194Trp genotype was statistically significant in relation to endometrial cancer susceptibility.

Dataset Information

A single nucleotide polymorphism of the interferon-? gene and susceptibility to hepatitis B virus-related cirrhosis: a randomized controlled trial.

Publications

A single nucleotide polymorphism of the interferon-γ gene and susceptibility to hepatitis B virus-related cirrhosis: a randomized controlled trial.

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