Dataset Information

A single nucleotide polymorphism of the interferon-? gene and susceptibility to hepatitis B virus-related cirrhosis: a randomized controlled trial.

ABSTRACT: OBJECTIVE:This study aimed to explore the correlation between a single nucleotide polymorphism (SNP) of the interferon-? (IFN-?) gene and susceptibility to hepatitis B virus (HBV) -related cirrhosis in the Chinese population. METHOD:PCR-LDR was employed for the genotyping of two SNPs, rs1861494 and rs2069718, of the IFN-? gene in 230 patients with HBV-related cirrhosis and 320 inactive HBsAg carriers without cirrhosis. It was then determined whether the Hardy-Weinberg (H-W) equilibrium was satisfied. The odds ratio (OR) and 95% confidence interval (CI) were analyzed using the chi-square test and univariate non-conditional logistic regression. Haplotypes were established using SHEsis and SNPStats online software and their interaction with non-genetic factors was analyzed. RESULTS:Compared with the AA genotype of rs1861494 SNP, AG+GG genotypes increased the risk of HBV-related cirrhosis. There was a significant difference in the distribution of A and G alleles between the case group and the control group (P<0.05). There was also a significant difference in the distribution of AA, AG, GG, AA, and AG+GG genotypes and A/G alleles between the case group and the control group (P<0.05). This indicated that the G allele may be a risk factor for HBV-related cirrhosis. By analyzing the different distribution of haplotypes in the case group and the control group, we observed significant differences in the distribution of AA, AG and GA haplotypes between the case and control groups (P<0.05). Haplotypes of the IFN-? gene did not interact with other relevant factors. CONCLUSION:The G allele of rs1861494 SNP as well as AG and GG genotypes and G allele of rs2069718 SNP may be risk factors of HBV-related cirrhosis. The AA haplotype may be a protective factor for HBV-related cirrhosis, while the AG haplotype is a risk factor for HBV-related cirrhosis.

SUBMITTER: Wang Q

PROVIDER: S-EPMC6962819 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

A single nucleotide polymorphism of the interferon-γ gene and susceptibility to hepatitis B virus-related cirrhosis: a randomized controlled trial.

Wang Qing Q Chang Fangzhi F Li Wei W

International journal of clinical and experimental pathology 20180801 8

<h4>Objective</h4>This study aimed to explore the correlation between a single nucleotide polymorphism (SNP) of the interferon-γ (IFN-γ) gene and susceptibility to hepatitis B virus (HBV) -related cirrhosis in the Chinese population.<h4>Method</h4>PCR-LDR was employed for the genotyping of two SNPs, rs1861494 and rs2069718, of the IFN-γ gene in 230 patients with HBV-related cirrhosis and 320 inactive HBsAg carriers without cirrhosis. It was then determined whether the Hardy-Weinberg (H-W) equili ...[more]

PMID: 31949806

Similar Datasets

Project description:BackgroundGenetic polymorphisms in the RAS gene family are associated with different diseases, which may include alcohol-related disorders. Previous studies showed an association of the allelic variant rs26907 in RASGRF2 gene with higher alcohol intake. Additionally, the rs61764370 polymorphism in the KRAS gene is located in a binding site for the let-7 micro-RNA family, which is potentially involved in alcohol-induced inflammation. Therefore, this study was designed to explore the association between these two polymorphisms and susceptibility to alcoholism or alcoholic liver disease (ALD).MethodsWe enrolled 301 male alcoholic patients and 156 healthy male volunteers in this study. Polymorphisms were genotyped by using TaqMan® PCR assays for allelic discrimination. Allelic and genotypic frequencies were compared between the two groups. Logistic regression analysis was performed to analyze the inheritance model.ResultsThe A allele of the RASGRF2 polymorphism (rs26907) was significantly more prevalent among alcoholic patients with cirrhosis (23.2%) compared to alcoholic patients without ALD (14.2%). This difference remained significant in the group of patients with alcohol dependence (28.8% vs. 14.3%) but not in those with alcohol abuse (15.1% vs. 14.4%). Multivariable logistic regression analysis showed that the A allele of this polymorphism (AA or GA genotype) was associated with alcoholic cirrhosis both in the total group of alcoholics (odds ratio [OR]: 2.33, 95% confidence interval [CI]: 1.32-4.11; P = 0.002) and in the group of patients with alcohol dependence (OR: 3.1, 95% CI: 1.50-6.20; P = 0.001). Allelic distributions of the KRAS polymorphism (rs61764370) did not differ between the groups.ConclusionsTo our knowledge, this genetic association study represents the first to show an association of the RASGRF2 G>A (rs26907) polymorphism with ALD in men, particularly in the subgroup of patients with AD. The findings suggest the potential relevance of the RAS gene family in alcoholism and ALD.

Dataset Information

A single nucleotide polymorphism of the interferon-? gene and susceptibility to hepatitis B virus-related cirrhosis: a randomized controlled trial.

Publications

A single nucleotide polymorphism of the interferon-γ gene and susceptibility to hepatitis B virus-related cirrhosis: a randomized controlled trial.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets