Ontology highlight
ABSTRACT:
SUBMITTER: Tan W
PROVIDER: S-EPMC7010609 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Tan Winnie W van Twest Sylvie S Murphy Vincent J VJ Deans Andrew J AJ
Frontiers in cell and developmental biology 20200204
DNA interstrand crosslinks (ICLs) are a physical barrier to replication and therefore toxic to cell viability. An important mechanism for the removal of ICLs is the Fanconi Anemia DNA repair pathway, which is initiated by mono-ubiquitination of FANCD2 and its partner protein FANCI. Here, we show that maintenance of FANCD2 and FANCI proteins in a monoubiquitinated form is regulated by the ATR-kinase. Using recombinant proteins in biochemical reconstitution experiments we show that ATR directly ph ...[more]