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Mapping Enzyme Activity on Tissue by Functional Mass Spectrometry Imaging.

ABSTRACT: Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High-resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high-resolution maps of phospholipase activity and specificity, which could subsequently be compared to histological images of the same section. Functional MSI thus represents a new and generalisable method for imaging biological activity in?situ.

SUBMITTER: Hamilton BR

PROVIDER: S-EPMC7106485 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Mapping Enzyme Activity on Tissue by Functional Mass Spectrometry Imaging.

Hamilton Brett R BR Marshall David L DL Casewell Nicholas R NR Harrison Robert A RA Blanksby Stephen J SJ Undheim Eivind A B EAB

Angewandte Chemie (International ed. in English) 20200123 10

Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High-resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high-reso ...[more]

PMID: 31854493

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