Ontology highlight
ABSTRACT:
SUBMITTER: Tan W
PROVIDER: S-EPMC7156235 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Tan Winnie W van Twest Sylvie S Leis Andrew A Bythell-Douglas Rohan R Murphy Vincent J VJ Sharp Michael M Parker Michael W MW Crismani Wayne W Deans Andrew J AJ
eLife 20200313
FANCI:FANCD2 monoubiquitination is a critical event for replication fork stabilization by the Fanconi anemia (FA) DNA repair pathway. It has been proposed that at stalled replication forks, monoubiquitinated-FANCD2 serves to recruit DNA repair proteins that contain ubiquitin-binding motifs. Here, we have reconstituted the FA pathway in vitro to study functional consequences of FANCI:FANCD2 monoubiquitination. We report that monoubiquitination does not promote any specific exogenous protein:prote ...[more]