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Magnetic Nanoparticles Behavior in Biological Solutions; The Impact of Clustering Tendency on Sedimentation Velocity and Cell Uptake.

ABSTRACT: Magnetic nanoparticles (MNPs) are prone to exhibit physicochemical changes caused by their interaction with biological solutions. However, such interactions have been less considered in cancer therapy studies. The behavior of four iron oxide MNP formulations with different surface coatings, namely, chitosan (CS), polyvinyl alcohol (PVA), carboxymethyldextran (CMX), and polydimethylamine (PEA), was investigated, after their exposure to four different cell culture media (DMEM/F12 and MEM, among others) and six different cancer cell lines (HT29, HT1080, T24, MDA-MB-231, BxPC-3, and LS174T). The sedimentation (Vs) and diffusion (Vd) velocities of MNPs in different culture media were calculated. Atomic absorption spectroscopy (AAS) and dynamic light scattering (DLS) were used to quantify cell uptake efficiency and physicochemical properties, respectively. Apart from PVA-coated MNPs, CMX-, CS-, and PEA-coated MNPs clustered and increased notably in size when dispensed in culture media. The different MNP formulations led either to a low (PVA-coated MNPs), medium (CS- and CMX-coated MNPs), or high (PEA-coated MNPs) clustering in the different culture media. Clustering correlated with the Vs and Vd of the MNPs and their subsequent interaction with cells. In particular, the CMX-coated MNPs with higher Vs and lower Vd internalized more readily than the PVA-coated MNPs into the different cell lines. Hence, our results highlight key considerations to include when validating nanoparticles for future biomedical applications.

SUBMITTER: Dabaghi M

PROVIDER: S-EPMC7178374 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Magnetic Nanoparticles Behavior in Biological Solutions; The Impact of Clustering Tendency on Sedimentation Velocity and Cell Uptake.

Dabaghi Mohammad M Hilger Ingrid I

Materials (Basel, Switzerland) 20200402 7

Magnetic nanoparticles (MNPs) are prone to exhibit physicochemical changes caused by their interaction with biological solutions. However, such interactions have been less considered in cancer therapy studies. The behavior of four iron oxide MNP formulations with different surface coatings, namely, chitosan (CS), polyvinyl alcohol (PVA), carboxymethyldextran (CMX), and polydimethylamine (PEA), was investigated, after their exposure to four different cell culture media (DMEM/F12 and MEM, among ot ...[more]

PMID: 32252307

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Project description:Magnetococcus marinus magnetosome-associated protein MamC, expressed as recombinant, has been proven to mediate the formation of novel biomimetic magnetic nanoparticles (BMNPs) that are successful drug nanocarriers for targeted chemotherapy and hyperthermia agents. These BMNPs present several advantages over inorganic magnetic nanoparticles, such as larger sizes that allow the former to have larger magnetic moment per particle, and an isoelectric point at acidic pH values, which allows both the stable functionalization of BMNPs at physiological pH value and the molecule release at acidic (tumor) environments, simply based on electrostatic interactions. However, difficulties for BMNPs cell internalization still hold back the efficiency of these nanoparticles as drug nanocarriers and hyperthermia agents. In the present study we explore the enhanced BMNPs internalization following upon their encapsulation by poly (lactic-co-glycolic) acid (PLGA), a Food and Drug Administration (FDA) approved molecule. Internalization is further optimized by the functionalization of the nanoformulation with the cell-penetrating TAT peptide (TATp). Our results evidence that cells treated with the nanoformulation [TAT-PLGA(BMNPs)] show up to 80% more iron internalized (after 72 h) compared to that of cells treated with BMNPs (40%), without any significant decrease in cell viability. This nanoformulation showing optimal internalization is further characterized. In particular, the present manuscript demonstrates that neither its magnetic properties nor its performance as a hyperthermia agent are significantly altered due to the encapsulation. In vitro experiments demonstrate that, following upon the application of an alternating magnetic field on U87MG cells treated with BMNPs and TAT-PLGA(BMNPs), the cytotoxic effect of BMNPs was not affected by the TAT-PLGA enveloping. Based on that, difficulties shown in previous studies related to poor cell uptake of BMNPs can be overcome by the novel nanoassembly described here.

Dataset Information

Magnetic Nanoparticles Behavior in Biological Solutions; The Impact of Clustering Tendency on Sedimentation Velocity and Cell Uptake.

Publications

Magnetic Nanoparticles Behavior in Biological Solutions; The Impact of Clustering Tendency on Sedimentation Velocity and Cell Uptake.

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