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SARS-CoV-2 Spike Protein Interacts with Multiple Innate Immune Receptors.

ABSTRACT: The spike (S) glycoprotein in the envelope of SARS-CoV-2 is densely glycosylated but the functions of its glycosylation are unknown. Here we demonstrate that S is recognized in a glycan-dependent manner by multiple innate immune receptors including the mannose receptor MR/CD206, DC-SIGN/CD209, L-SIGN/CD209L, and MGL/CLEC10A/CD301. Single-cell RNA sequencing analyses indicate that such receptors are highly expressed in innate immune cells in tissues susceptible to SARS-CoV-2 infection. Binding of the above receptors to S is characterized by affinities in the picomolar range and consistent with S glycosylation analysis demonstrating a variety of N- and O-glycans as receptor ligands. These results indicate multiple routes for SARS-CoV-2 to interact with human cells and suggest alternative strategies for therapeutic intervention.

SUBMITTER: Gao C

PROVIDER: S-EPMC7402034 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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SARS-CoV-2 Spike Protein Interacts with Multiple Innate Immune Receptors.

Gao Chao C Zeng Junwei J Jia Nan N Stavenhagen Kathrin K Matsumoto Yasuyuki Y Zhang Hua H Li Jiang J Hume Adam J AJ Mühlberger Elke E van Die Irma I Kwan Julian J Tantisira Kelan K Emili Andrew A Cummings Richard D RD

bioRxiv : the preprint server for biology 20200730

The spike (S) glycoprotein in the envelope of SARS-CoV-2 is densely glycosylated but the functions of its glycosylation are unknown. Here we demonstrate that S is recognized in a glycan-dependent manner by multiple innate immune receptors including the mannose receptor MR/CD206, DC-SIGN/CD209, L-SIGN/CD209L, and MGL/CLEC10A/CD301. Single-cell RNA sequencing analyses indicate that such receptors are highly expressed in innate immune cells in tissues susceptible to SARS-CoV-2 infection. Binding of ...[more]

PMID: 32766577

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