Dataset Information

LncRNA and mRNA expression profiles and functional networks of hyposalivation of the submandibular gland in hypertension.

ABSTRACT: Hyposalivation is a complication of hypertension. However, little is known about the role of long non-coding RNAs (lncRNAs) in salivary glands in hypertension. This study aimed to compare the lncRNA and mRNA expression profiles between spontaneous hypertension rats (SHRs) and Wistar-Kyoto (WKY) rats through microarray analysis and apple bioinformatics methods to analyse their potential roles in hyposalivation. The differentially expressed (DE) lncRNAs and mRNAs were confirmed by quantitative real-time PCR (qRT-PCR). Compared with WKY rats, 225 DE lncRNAs and 473 DE mRNAs were identified in the SMG of SHRs. The pathway analyses of DE mRNAs showed that inflammatory mediator regulation of transient receptor potential channels was involved in hyposalivation in SHRs. Ten DE lncRNAs were chosen for further research. A coding-non-coding gene co-expression (CNC) network and competing endogenous RNA (ceRNA) network analysis revealed that the potential functions of these 10 DE lncRNAs were closely connected with the processes of the immune response. This study showed abundant DE lncRNAs and mRNAs in hypertensive SMGs. Furthermore, our results indicated strong associations between the immune response and hyposalivation and showed the potential of immune-related genes as novel and therapeutic targets for hyposalivation.

SUBMITTER: Shen ZJ

PROVIDER: S-EPMC7434885 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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LncRNA and mRNA expression profiles and functional networks of hyposalivation of the submandibular gland in hypertension.

Shen Zhu-Jun ZJ Han Ye-Chen YC Wang Yi-Ning YN Xie Hong-Zhi HZ

Scientific reports 20200818 1

Hyposalivation is a complication of hypertension. However, little is known about the role of long non-coding RNAs (lncRNAs) in salivary glands in hypertension. This study aimed to compare the lncRNA and mRNA expression profiles between spontaneous hypertension rats (SHRs) and Wistar-Kyoto (WKY) rats through microarray analysis and apple bioinformatics methods to analyse their potential roles in hyposalivation. The differentially expressed (DE) lncRNAs and mRNAs were confirmed by quantitative rea ...[more]

PMID: 32811845

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Project description:BACKGROUND:The goals of this study were to explore the expression profiles and functional networks of long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in mesenchymal stromal cells (MSCs) involved in regulating the function of monocytes and to clarify the mechanisms by which MSCs exert immunoregulatory effects on monocytes. METHODS:MSCs and CD14+ monocytes were separately isolated. The immunoregulatory effects of MSCs on monocytes were determined by flow cytometry. lncRNAs and mRNAs that were differentially expressed (DE) between the control group (MSCs only) and co-culture group (MSCs co-cultured with monocytes) were identified through high-throughput sequencing and bioinformatic analyses and were confirmed by qRT-PCR. Bioinformatic analyses were performed to identify the critical biological functions and signalling pathways involved in MSC-mediated monocyte regulation and to identify the functional networks formed between DE mRNAs and lncRNAs. RESULTS:MSCs showed a strong ability to induce monocyte migration but inhibited monocyte differentiation into M1 macrophages. A total of 145 DE lncRNAs and 768 DE mRNAs were identified between the control and co-culture groups. Significant fold changes in lncRNAs and mRNAs were confirmed by qRT-PCR. GO analysis demonstrated that DE mRNAs and lncRNAs were highly associated with terms related to binding and biological regulation. KEGG analysis revealed 122 significantly regulated pathways, including the cytokine-cytokine receptor pathway and chemokine signalling pathway. Interaction and co-expression networks were constructed for DE mRNAs and lncRNAs, and several key microRNAs were identified in the competitive endogenous RNA (ceRNA) network. Target genes of the DE lncRNAs were analysed to predict critical mRNA-lncRNA axes involved in the immunoregulatory function of MSCs. CONCLUSIONS:Our research describes the lncRNA and mRNA expression profiles and functional networks involved in MSC-mediated regulation of monocytes. These results provide possible molecular mechanisms for the immunoregulatory function of MSCs and may help to elucidate possible molecular therapeutic targets in MSCs for the treatment of autoimmune diseases.

Project description:Abstract: Background: Hyposalivation is one of the common symptoms of diabetes. Although the micro ribonucleic acid (miRNA) has recently been identified to play a role in the pathogenesis of diabetes, the specific effects of miRNAs on diabetic salivary glands remain unclear. This work was aimed to investigate the expression profiles of miRNAs and identify miRNA‐mRNA network in submandibular gland (SMG) in db/db mice. Methods: miRNA and mRNA expressional profiles were analyzed by microarray technology from the SMG of db/db mice and db/m mice. Significant fold changes in miRNAs and mRNAs were confirmed by real-time quantitative reverse transcription-polymerase chain reaction. Bioinformatic analyses were performed to identify the critical biological functions and signaling pathways involved in diabetes-mediated hyposalivation and to identify the functional networks formed between differentially expressed (DE) miRNAs and mRNAs. Results: A total of 28 DE miRNAs and 1146 DE mRNAs were identified of the SMG in db/db mice and db/m mice. Gene ontology (GO) analysis and KEGG pathway analysis demonstrated that DE miRNAs were highly associated with terms related to diverse biological processes and signaling pathways. Of the related pathways, TGF-beta signaling pathway and tight junction pathway were notable. The constructed network indicated interactions between 11 miRNAs and 42 mRNAs. Autophagy pathway is worth studying, especially the dynamic network of miRNA and mRNA. In addition, AKT3 and PIK3CD may play important roles in the development of diabetes-mediated hyposalivation. Conclusions: Our research describes the miRNA and mRNA expression profiles and functional networks involved in SMGs from db/db mice. These results provide possible molecular mechanisms and may help to elucidate possible molecular therapeutic targets for the treatment of diabetes-induced hyposalivation. Key words: diabetes, submandibular gland, miRNA, mRNA, miRNA–mRNA network, microarray

Dataset Information

LncRNA and mRNA expression profiles and functional networks of hyposalivation of the submandibular gland in hypertension.

Publications

LncRNA and mRNA expression profiles and functional networks of hyposalivation of the submandibular gland in hypertension.

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