Ontology highlight
ABSTRACT:
SUBMITTER: Sutton G
PROVIDER: S-EPMC7477198 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Sutton Geoff G Sun Dapeng D Fu Xiaofeng X Kotecha Abhay A Hecksel Corey W CW Clare Daniel K DK Zhang Peijun P Stuart David I DI Boyce Mark M
Nature communications 20200907 1
Traditionally, molecular assembly pathways for viruses are inferred from high resolution structures of purified stable intermediates, low resolution images of cell sections and genetic approaches. Here, we directly visualise an unsuspected 'single shelled' intermediate for a mammalian orthoreovirus in cryo-preserved infected cells, by cryo-electron tomography of cellular lamellae. Particle classification and averaging yields structures to 5.6 Å resolution, sufficient to identify secondary struct ...[more]