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Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow.

ABSTRACT: A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5-11 and space-time yields of up to 22.9 g L-1 h-1. The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation.

SUBMITTER: Poznansky B

PROVIDER: S-EPMC7574627 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow.

Poznansky Barnabas B Thompson Lisa A LA Warren Sarah A SA Reeve Holly A HA Vincent Kylie A KA

Organic process research & development 20200131 10

A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5-11 and space-time yields of up to 22.9 g L<sup>-1</sup> h<sup>-1</sup> ...[more]

PMID: 33100814

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