Unknown

Dataset Information

0

Highly sensitive serological approaches for Pepino mosaic virus detection.


ABSTRACT: Pepino mosaic virus (PepMV) causes severe disease in tomato and other Solanaceous crops around globe. To effectively study and manage this viral disease, researchers need new, sensitive, and high-throughput approaches for viral detection. In this study, we purified PepMV particles from the infected Nicotiana benthamiana plants and used virions to immunize BALB/c mice to prepare hybridomas secreting anti-PepMV monoclonal antibodies (mAbs). A panel of highly specific and sensitive murine mAbs (15B2, 8H6, 23D11, 20D9, 3A6, and 8E3) could be produced through cell fusion, antibody selection, and cell cloning. Using the mAbs as the detection antibodies, we established double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), Dot-ELISA, and Tissue print-ELISA for detecting PepMV infection in tomato plants. Resulting data on sensitivity analysis assays showed that both DAS-ELISA and Dot-ELISA can efficiently monitor the virus in PepMV-infected tissue crude extracts when diluted at 1:1 310 720 and 1:20 480 (weight/volume ratio (w/v), g/mL), respectively. Among the three methods developed, the Tissue print-ELISA was found to be the most practical detection technique. Survey results from field samples by the established serological approaches were verified by reverse transcription polymerase chain reaction (RT-PCR) and DNA sequencing, demonstrating all three serological methods are reliable and effective for monitoring PepMV. Anti-PepMV mAbs and the newly developed DAS-ELISA, Dot-ELISA, and Tissue print-ELISA can benefit PepMV detection and field epidemiological study, and management of this viral disease, which is already widespread in tomato plants in Yunnan Province of China.

SUBMITTER: He WQ 

PROVIDER: S-EPMC7606197 | biostudies-literature | 2020 Oct.

REPOSITORIES: biostudies-literature

altmetric image

Publications

Highly sensitive serological approaches for Pepino mosaic virus detection.

He Wan-Qin WQ   Wu Jia-Yu JY   Ren Yi-Yi YY   Zhou Xue-Ping XP   Zhang Song-Bai SB   Qian Ya-Juan YJ   Li Fang-Fang FF   Wu Jian-Xiang JX  

Journal of Zhejiang University. Science. B 20201001 10


Pepino mosaic virus (PepMV) causes severe disease in tomato and other Solanaceous crops around globe. To effectively study and manage this viral disease, researchers need new, sensitive, and high-throughput approaches for viral detection. In this study, we purified PepMV particles from the infected Nicotiana benthamiana plants and used virions to immunize BALB/c mice to prepare hybridomas secreting anti-PepMV monoclonal antibodies (mAbs). A panel of highly specific and sensitive murine mAbs (15B  ...[more]

Similar Datasets

| S-EPMC9409097 | biostudies-literature
2022-05-24 | GSE203627 | GEO
| S-EPMC3957916 | biostudies-literature
| S-EPMC6485256 | biostudies-literature
| S-EPMC8290496 | biostudies-literature
| S-EPMC7159214 | biostudies-literature
| S-EPMC6952647 | biostudies-literature
| S-EPMC5339997 | biostudies-literature
| S-EPMC7472245 | biostudies-literature
| S-EPMC2786733 | biostudies-literature