Unknown

Dataset Information

0

NMR and Computational Studies as Analytical and High-Resolution Structural Tool for Complex Hydroperoxides and Diastereomeric Endo-Hydroperoxides of Fatty Acids in Solution-Exemplified by Methyl Linolenate.


ABSTRACT: A combination of selective 1D Total Correlation Spectroscopy (TOCSY) and 1H-13C Heteronuclear Multiple Bond Correlation (HMBC) NMR techniques has been employed for the identification of methyl linolenate primary oxidation products without the need for laborious isolation of the individual compounds. Complex hydroperoxides and diastereomeric endo-hydroperoxides were identified and quantified. Strongly deshielded C-O-O-H 1H-NMR resonances of diastereomeric endo-hydroperoxides in the region of 8.8 to 9.6 ppm were shown to be due to intramolecular hydrogen bonding interactions of the hydroperoxide proton with an oxygen atom of the five-member endo-peroxide ring. These strongly deshielded resonances were utilized as a new method to derive, for the first time, three-dimensional structures with an assignment of pairs of diastereomers in solution with the combined use of 1H-NMR chemical shifts, Density Functional Theory (DFT), and Our N-layered Integrated molecular Orbital and molecular Mechanics (ONIOM) calculations.

SUBMITTER: Ahmed R 

PROVIDER: S-EPMC7660186 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

NMR and Computational Studies as Analytical and High-Resolution Structural Tool for Complex Hydroperoxides and Diastereomeric <i>Endo</i>-Hydroperoxides of Fatty Acids in Solution-Exemplified by Methyl Linolenate.

Ahmed Raheel R   Varras Panayiotis C PC   Siskos Michael G MG   Siddiqui Hina H   Choudhary M Iqbal MI   Gerothanassis Ioannis P IP  

Molecules (Basel, Switzerland) 20201023 21


A combination of selective 1D Total Correlation Spectroscopy (TOCSY) and <sup>1</sup>H-<sup>13</sup>C Heteronuclear Multiple Bond Correlation (HMBC) NMR techniques has been employed for the identification of methyl linolenate primary oxidation products without the need for laborious isolation of the individual compounds. Complex hydroperoxides and diastereomeric <i>endo</i>-hydroperoxides were identified and quantified. Strongly deshielded C-O-O-H <sup>1</sup>H-NMR resonances of diastereomeric <  ...[more]

Similar Datasets

| S-EPMC4084788 | biostudies-literature
| S-EPMC7508003 | biostudies-literature
| S-EPMC10456989 | biostudies-literature
| S-EPMC7663419 | biostudies-literature
| S-EPMC2916639 | biostudies-literature
| S-EPMC6151582 | biostudies-literature
| S-EPMC2846706 | biostudies-literature
| S-EPMC5256481 | biostudies-literature
| S-EPMC3807244 | biostudies-literature
2009-06-02 | E-GEOD-14696 | biostudies-arrayexpress