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Distinct oligodendrocyte populations have spatial preference and different responses to spinal cord injury.

ABSTRACT: Mature oligodendrocytes (MOLs) show transcriptional heterogeneity, the functional consequences of which are unclear. MOL heterogeneity might correlate with the local environment or their interactions with different neuron types. Here, we show that distinct MOL populations have spatial preference in the mammalian central nervous system (CNS). We found that MOL type 2 (MOL2) is enriched in the spinal cord when compared to the brain, while MOL types 5 and 6 (MOL5/6) increase their contribution to the OL lineage with age in all analyzed regions. MOL2 and MOL5/6 also have distinct spatial preference in the spinal cord regions where motor and sensory tracts run. OL progenitor cells (OPCs) are not specified into distinct MOL populations during development, excluding a major contribution of OPC intrinsic mechanisms determining MOL heterogeneity. In disease, MOL2 and MOL5/6 present different susceptibility during the chronic phase following traumatic spinal cord injury. Our results demonstrate that the distinct MOL populations have different spatial preference and different responses to disease.

SUBMITTER: Floriddia EM

PROVIDER: S-EPMC7673029 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Distinct oligodendrocyte populations have spatial preference and different responses to spinal cord injury

Project description:In this study, we unveil that transcriptionally distinct oligodendrocyte populations have spatial preference and differential response to spinal cord injury. We also assess by different methods, including single-cell RNA sequencing, that the developmental origin of oligodendrocyte progenitors does not determine their specification into mature olgodendrocyte populations.

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Distinct oligodendrocyte populations have spatial preference and different responses to spinal cord injury

Project description:Distinct oligodendrocyte populations have spatial preference and different responses to spinal cord injury

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