Dataset Information
In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil.
Ontology highlight
ABSTRACT:
Methods: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays.
Results: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences.
Conclusion: These results should be taken into consideration when using PCR-based diagnostics in Brazil.
SUBMITTER: Santos RDS
PROVIDER: S-EPMC7723368 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil.
Revista da Sociedade Brasileira de Medicina Tropical 20201125
<h4>Introduction</h4>Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results.<h4>Methods</h4>Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays.<h4>Results</h4>Only 3 of the 15 completely ali ...[more]
