Ontology highlight
ABSTRACT:
SUBMITTER: Escudero JA
PROVIDER: S-EPMC7790495 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Escudero José Antonio JA Nivina Aleksandra A Kemble Harry E HE Loot Céline C Tenaillon Olivier O Mazel Didier D
eLife 20201215
Molecular examples of evolutionary innovation are scarce and generally involve point mutations. Innovation can occur through larger rearrangements, but here experimental data is extremely limited. Integron integrases innovated from double-strand- toward single-strand-DNA recombination through the acquisition of the I2 α-helix. To investigate how this transition was possible, we have evolved integrase IntI1 to what should correspond to an early innovation state by selecting for its ancestral acti ...[more]