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Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue.

ABSTRACT: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized nuclei/cell sorting from mouse or human embryonic cortical tissue for distinct downstream molecular investigation of basal intermediate progenitors.

SUBMITTER: Sakib MS

PROVIDER: S-EPMC7859298 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue.

Sakib M Sadman MS Sokpor Godwin G Nguyen Huu Phuc HP Fischer Andre A Tuoc Tran T

STAR protocols 20210202 1

Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized ...[more]

PMID: 33554149

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