Browse
Submit Data
Databases
API
Help

Dataset Information

24 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Micron-sized particle separation with standing surface acoustic wave-Experimental and numerical approaches.

ABSTRACT: Traditional cell/particle isolation methods are time-consuming and expensive and can lead to morphology disruptions due to high induced shear stress. To address these problems, novel lab-on-a-chip-based purification methods have been employed. Among various methods introduced for the separation and purification of cells and synthetics particles, acoustofluidics has been one of the most effective methods. Unlike traditional separation techniques carried out in clinical laboratories based on chemical properties, the acoustofluidic process relies on the physical properties of the sample. Using acoustofluidics, manipulating cells and particles can be achieved in a label-free, contact-free, and highly biocompatible manner. To optimize the functionality of the platform, the numerical study should be taken into account before conducting experimental tests to save time and reduce fabrication expenses. Most current numerical studies have only considered one-dimensional harmonic standing waves to simulate the acoustic pressure distribution. However, one-dimensional simulations cannot calculate the actual acoustic pressure distribution inside the microchannel due to its limitation in considering longitudinal waves. To address this limitation, a two-dimensional numerical simulation was conducted in this study. Our numerical simulation investigates the effects of the platform geometrical and operational conditions on the separation efficiency. Next, the optimal values are tested in an experimental setting to validate these optimal parameters and conditions. This work provides a guideline for future acoustofluidic chip designs with a high degree of reproducibility and efficiency.

SUBMITTER: Taatizadeh E

PROVIDER: S-EPMC8267599 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Experimental and numerical studies on standing surface acoustic wave microfluidics.

Project description:Standing surface acoustic waves (SSAW) are commonly used in microfluidics to manipulate cells and other micro/nano particles. However, except for a simple one-dimensional (1D) harmonic standing waves (HSW) model, a practical model that can predict particle behaviour in SSAW microfluidics is still lacking. Herein, we established a two-dimensional (2D) SSAW microfluidic model based on the basic theory in acoustophoresis and our previous modelling strategy to predict the acoustophoresis of microparticles in SSAW microfluidics. This 2D SSAW microfluidic model considers the effects of boundary vibrations, channel materials, and channel dimensions on the acoustic propagation; as an experimental validation, the acoustophoresis of microparticles under continuous flow through narrow channels made of PDMS and silicon was studied. The experimentally observed motion of the microparticles matched well with the numerical predictions, while the 1D HSW model failed to predict many of the experimental observations. Particularly, the 1D HSW model cannot account for particle aggregation on the sidewall in PDMS channels, which is well explained by our 2D SSAW microfluidic model. Our model can be used for device design and optimization in SSAW microfluidics.

| S-EPMC4856433 | biostudies-literature

3D numerical simulation of acoustophoretic motion induced by boundary-driven acoustic streaming in standing surface acoustic wave microfluidics.

Project description:Standing surface acoustic waves (SSAWs) have been widely utilized in microfluidic devices to manipulate various cells and micro/nano-objects. Despite widespread application, a time-/cost-efficient versatile 3D model that predicts particle behavior in such platforms is still lacking. Herein, a fully-coupled 3D numerical simulation of boundary-driven acoustic streaming in the acoustofluidic devices utilizing SSAWs has been conducted based on the limiting velocity finite element method. Through this efficient computational method, the underlying physical interplay from the electromechanical fields of the piezoelectric substrate to different acoustofluidic effects (acoustic radiation force and streaming-induced drag force), fluid-solid interactions, the 3D influence of novel on-chip configuration like tilted-angle SSAW (taSSAW) based devices, required boundary conditions, meshing technique, and demanding computational cost, are discussed. As an experimental validation, a taSSAW platform fabricated on YX 128 [Formula: see text] LiNbO3 substrate for separating polystyrene beads is simulated, which demonstrates acceptable agreement with reported experimental observations. Subsequently, as an application of the presented 3D model, a novel sheathless taSSAW cell/particle separator is conceptualized and designed. The presented 3D fully-coupled model could be considered a powerful tool in further designing and optimizing SSAW microfluidics due to the more time-/cost-efficient performance than precedented 3D models, the capability to model complex on-chip configurations, and overcome shortcomings and limitations of 2D simulations.

| S-EPMC8233446 | biostudies-literature

Standing surface acoustic wave based cell coculture.

Project description:Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.

| S-EPMC4188268 | biostudies-literature

Numerical Determination of the Secondary Acoustic Radiation Force on a Small Sphere in a Plane Standing Wave Field.

Project description:Two numerical methods based on the Finite Element Method are presented for calculating the secondary acoustic radiation force between interacting spherical particles. The first model only considers the acoustic waves scattering off a single particle, while the second model includes re-scattering effects between the two interacting spheres. The 2D axisymmetric simplified model combines the Gor'kov potential approach with acoustic simulations to find the interacting forces between two small compressible spheres in an inviscid fluid. The second model is based on 3D simulations of the acoustic field and uses the tensor integral method for direct calculation of the force. The results obtained by both models are compared with analytical equations, showing good agreement between them. The 2D and 3D models take, respectively, seconds and tens of seconds to achieve a convergence error of less than 1%. In comparison with previous models, the numerical methods presented herein can be easily implemented in commercial Finite Element software packages, where surface integrals are available, making it a suitable tool for investigating interparticle forces in acoustic manipulation devices.

| S-EPMC6680761 | biostudies-literature

Investigation into the Effect of Acoustic Radiation Force and Acoustic Streaming on Particle Patterning in Acoustic Standing Wave Fields.

Project description:Acoustic standing waves have been widely used in trapping, patterning, and manipulating particles, whereas one barrier remains: the lack of understanding of force conditions on particles which mainly include acoustic radiation force (ARF) and acoustic streaming (AS). In this paper, force conditions on micrometer size polystyrene microspheres in acoustic standing wave fields were investigated. The COMSOL® Mutiphysics particle tracing module was used to numerically simulate force conditions on various particles as a function of time. The velocity of particle movement was experimentally measured using particle imaging velocimetry (PIV). Through experimental and numerical simulation, the functions of ARF and AS in trapping and patterning were analyzed. It is shown that ARF is dominant in trapping and patterning large particles while the impact of AS increases rapidly with decreasing particle size. The combination of using both ARF and AS for medium size particles can obtain different patterns with only using ARF. Findings of the present study will aid the design of acoustic-driven microfluidic devices to increase the diversity of particle patterning.

| S-EPMC5539529 | biostudies-other

Separation of sub-micron particles from micron particles using acoustic fluid relocation combined with acoustophoresis.

Project description:Acoustophoresis has gained increasing attention as a gentle, non-contact, and high-throughput cell and particle separation technique. It is conveniently used to isolate and enrich particles that are greater than 2 ?m; however, its use in manipulating particles smaller than 2 ?m is limited. In this work, we present an alternative way of using acoustic forces to manipulate sub-micrometer particles in continuous flow fashion. It has been shown that acoustic forces can be employed to relocate parallel laminar flow streams of two impedance-mismatched fluids. We demonstrate the separation of sub-micron particles from micron particles by the combination of acoustophoresis and acoustic fluid relocation. The micron particles are focused into the middle of the flow channel via primary acoustic forces while sub-micron particles are moved to the side via drag forces created by the relocating fluid. We demonstrate the proof of the concept using binary mixtures of particles comprised of sub-micron/micron particles, micron/micron particles, and bovine red blood cells with E. coli. The efficiency of the particle enrichment is determined via flow cytometry analysis of the collected streams. This study demonstrates that by combining acoustic fluid relocation with acoustophoresis, sub-micron particles can be effectively separated from micron particles at high flow rates and it can be further implemented to separate binary mixtures of micron particles if the volumetric ratio of two particles is greater than 10 and the larger particle diameter is about 10 ?m. The combined method is more appropriate to use than acoustophoresis in situations where acoustic streaming and differences in acoustic impedance of fluids can be of concern. Graphical abstract In the presence of a resonance acoustic field, the clean high-density fluid (dark gray) and the low-density sample fluid are relocated. During this process, E. coli are separated from the red blood cells (RBCs).

| S-EPMC6143349 | biostudies-literature

Standing surface acoustic wave (SSAW)-based cell washing.

Project description:Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing surface acoustic wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing surface acoustic wave (taSSAW) is employed in our device, enabling us to wash beads with >98% recovery rate and >97% washing efficiency. We also demonstrate the functionality of our device by preparing high-purity (>97%) white blood cells from lysed blood samples through cell washing. Our SSAW-based cell/bead washing device has the advantages of label-free manipulation, simplicity, high biocompatibility, high recovery rate, and high washing efficiency. It can be useful for many lab-on-a-chip applications.

| S-EPMC4442640 | biostudies-literature

Standing surface acoustic wave (SSAW)-based microfluidic cytometer.

Project description:The development of microfluidic chip-based cytometers has become an important area due to their advantages of compact size and low cost. Herein, we demonstrate a sheathless microfluidic cytometer which integrates a standing surface acoustic wave (SSAW)-based microdevice capable of 3D particle/cell focusing with a laser-induced fluorescence (LIF) detection system. Using SSAW, our microfluidic cytometer was able to continuously focus microparticles/cells at the pressure node inside a microchannel. Flow cytometry was successfully demonstrated using this system with a coefficient of variation (CV) of less than 10% at a throughput of ~1000 events s(-1) when calibration beads were used. We also demonstrated that fluorescently labeled human promyelocytic leukemia cells (HL-60) could be effectively focused and detected with our SSAW-based system. This SSAW-based microfluidic cytometer did not require any sheath flows or complex structures, and it allowed for simple operation over a wide range of sample flow rates. Moreover, with the gentle, bio-compatible nature of low-power surface acoustic waves, this technique is expected to be able to preserve the integrity of cells and other bioparticles.

| S-EPMC3956078 | biostudies-literature

Cell separation using tilted-angle standing surface acoustic waves.

Project description:Separation of cells is a critical process for studying cell properties, disease diagnostics, and therapeutics. Cell sorting by acoustic waves offers a means to separate cells on the basis of their size and physical properties in a label-free, contactless, and biocompatible manner. The separation sensitivity and efficiency of currently available acoustic-based approaches, however, are limited, thereby restricting their widespread application in research and health diagnostics. In this work, we introduce a unique configuration of tilted-angle standing surface acoustic waves (taSSAW), which are oriented at an optimally designed inclination to the flow direction in the microfluidic channel. We demonstrate that this design significantly improves the efficiency and sensitivity of acoustic separation techniques. To optimize our device design, we carried out systematic simulations of cell trajectories, matching closely with experimental results. Using numerically optimized design of taSSAW, we successfully separated 2- and 10-µm-diameter polystyrene beads with a separation efficiency of ∼ 99%, and separated 7.3- and 9.9-µm-polystyrene beads with an efficiency of ∼ 97%. We illustrate that taSSAW is capable of effectively separating particles-cells of approximately the same size and density but different compressibility. Finally, we demonstrate the effectiveness of the present technique for biological-biomedical applications by sorting MCF-7 human breast cancer cells from nonmalignant leukocytes, while preserving the integrity of the separated cells. The method introduced here thus offers a unique route for separating circulating tumor cells, and for label-free cell separation with potential applications in biological research, disease diagnostics, and clinical practice.

| S-EPMC4246961 | biostudies-literature

Three-dimensional numerical simulation and experimental investigation of boundary-driven streaming in surface acoustic wave microfluidics.

Project description:Acoustic streaming has been widely used in microfluidics to manipulate various micro-/nano-objects. In this work, acoustic streaming activated by interdigital transducers (IDT) immersed in highly viscous oil is studied numerically and experimentally. In particular, we developed a modeling strategy termed the "slip velocity method" that enables a 3D simulation of surface acoustic wave microfluidics in a large domain (4 × 4 × 2 mm3) and at a high frequency (23.9 MHz). The experimental and numerical results both show that on top of the oil, all the acoustic streamlines converge at two horizontal stagnation points above the two symmetric sides of the IDT. At these two stagnation points, water droplets floating on the oil can be trapped. Based on these characteristics of the acoustic streaming field, we designed a surface acoustic wave microfluidic device with an integrated IDT array fabricated on a 128°YX LiNbO3 substrate to perform programmable, contactless droplet manipulation. By activating IDTs accordingly, the water droplets on the oil can be moved to the corresponding traps. With its excellent capability for manipulating droplets in a highly programmable, controllable manner, our surface acoustic wave microfluidic devices are valuable for on-chip contactless sample handling and chemical reactions.

| S-EPMC6291338 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data