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Standing surface acoustic wave based cell coculture.

ABSTRACT: Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.

SUBMITTER: Li S

PROVIDER: S-EPMC4188268 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Standing surface acoustic wave based cell coculture.

Li Sixing S Guo Feng F Chen Yuchao Y Ding Xiaoyun X Li Peng P Wang Lin L Cameron Craig E CE Huang Tony Jun TJ

Analytical chemistry 20140918 19

Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cel ...[more]

PMID: 25232648

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