Unknown

Dataset Information

0

Dual Processing of R-Loops and Topoisomerase I Induces Transcription-Dependent DNA Double-Strand Breaks.


ABSTRACT: Although accumulation of DNA damage and genomic instability in resting cells can cause neurodegenerative disorders, our understanding of how transcription produces DNA double-strand breaks (DSBs) is limited. Transcription-blocking topoisomerase I cleavage complexes (TOP1ccs) are frequent events that prime DSB production in non-replicating cells. Here, we report a mechanism of their formation by showing that they arise from two nearby single-strand breaks (SSBs) on opposing DNA strands: one SSB from the removal of transcription-blocking TOP1ccs by the TDP1 pathway and the other from the cleavage of R-loops by endonucleases, including XPF, XPG, and FEN1. Genetic defects in TOP1cc removal (TDP1, PNKP, and XRCC1) or in the resolution of R-loops (SETX) enhance DSB formation and prevent their repair. Such deficiencies cause neurological disorders. Owing to the high frequency of TOP1cc trapping and the widespread distribution of R-loops, these persistent transcriptional DSBs could accumulate over time in neuronal cells, contributing to the neurodegenerative diseases.

SUBMITTER: Cristini A 

PROVIDER: S-EPMC8274950 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2726680 | biostudies-literature
| S-EPMC7079294 | biostudies-literature
| S-EPMC2857040 | biostudies-literature
| S-EPMC2920610 | biostudies-literature
| S-EPMC5286372 | biostudies-literature
| S-EPMC6037730 | biostudies-literature
| S-EPMC4826256 | biostudies-literature
2015-01-31 | E-GEOD-62927 | biostudies-arrayexpress
2018-02-20 | E-MTAB-6318 | biostudies-arrayexpress
2015-01-31 | GSE62927 | GEO