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Cinnamomum cassia and Syzygium aromaticum Essential Oils Reduce the Colonization of Salmonella Typhimurium in an In Vivo Infection Model Using Caenorhabditis elegans.


ABSTRACT: The regulation of intestinal colonization in livestock by means of non-bactericidal additives is an important management lever for zoonotic bacteria such as Salmonella spp. Caenorhabditis elegans is proposed here as a model for the evaluation of five essential oils (EOs) as anti-colonization products against Salmonella Typhimurium. An evaluation of the toxicity of EOs for C. elegans showed LD50 values ranging from 74.5 ± 9.6 µg/mL for Cinnamomum cassia (CEO) to 271.6 ± 14.9 µg/mL for Syzygium aromaticum (SyEO). Both EOs significantly inhibited bacterial colonization in the digestive tract of C. elegans with reductions of 0.88 and 0.70 log CFU/nematode at nontoxic concentrations of 50 µg/mL and 150 µg/mL, respectively. With the minimal bactericidal concentrations of CEO and SyEO against S. Typhimurium being 312.5 µg/mL and 625 µg/mL, respectively, an antibacterial effect can be excluded to explain the inhibition of the bacterial load. The anti-colonizing activity of these two EOs could, however, be related to an inhibition of the swimming motility, which was significantly reduced by 23.47% for CEO at 50 µg/mL and 19.56% for SyEO at 150 µg/mL. This study shows the potential of C. elegans as a predictive in vivo model of anti-colonizing activities that is suitable for the evaluation of essential oils.

SUBMITTER: Lang M 

PROVIDER: S-EPMC8467367 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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<i>Cinnamomum cassia</i> and <i>Syzygium aromaticum</i> Essential Oils Reduce the Colonization of <i>Salmonella</i> Typhimurium in an In Vivo Infection Model Using <i>Caenorhabditis elegans</i>.

Lang Marie M   Montjarret Aude A   Duteil Emmanuel E   Bedoux Gilles G  

Molecules (Basel, Switzerland) 20210915 18


The regulation of intestinal colonization in livestock by means of non-bactericidal additives is an important management lever for zoonotic bacteria such as <i>Salmonella</i> spp. <i>Caenorhabditis elegans</i> is proposed here as a model for the evaluation of five essential oils (EOs) as anti-colonization products against <i>Salmonella</i> Typhimurium. An evaluation of the toxicity of EOs for <i>C. elegans</i> showed LD<sub>50</sub> values ranging from 74.5 ± 9.6 µg/mL for <i>Cinnamomum cassia</  ...[more]

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