Unknown

Dataset Information

0

Protocol for cell type-specific labeling, enrichment, and proteomic profiling of plasma proteins in mice.


ABSTRACT: Secreted polypeptides represent a fundamental axis of intercellular communication. Here, we present a protocol for the cell type-specific biotinylation, enrichment, and proteomic profiling of secreted plasma proteins directly in mice. This protocol uses conditional "turn-on" adeno-associated viruses expressing an endoplasmic reticulum-targeted biotin ligase to globally biotinylate proteins of the secretory pathway in a cell type-specific manner. Biotinylated secreted proteins can be directly purified from blood plasma and analyzed by SDS-PAGE gel or shotgun proteomics. For complete information on the generation and use of this protocol, please refer to Wei et al. (2021).

SUBMITTER: Wei W 

PROVIDER: S-EPMC8672099 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC9742329 | biostudies-literature
| S-EPMC8941215 | biostudies-literature
| S-EPMC3887783 | biostudies-literature
| S-EPMC6270137 | biostudies-literature
| S-EPMC2731429 | biostudies-literature
| S-EPMC10497999 | biostudies-literature
| S-EPMC10548608 | biostudies-literature
| S-EPMC5813412 | biostudies-literature
| S-EPMC5553119 | biostudies-literature
2022-02-28 | PXD030663 | Pride