Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells.

ABSTRACT: Efficient translation of human induced pluripotent stem cells (hiPSCs) requires scalable cell manufacturing strategies for optimal self-renewal and functional differentiation. Traditional manual cell culture is variable and labor intensive, posing challenges for high-throughput applications. Here, we established a robotic platform and automated all essential steps of hiPSC culture and differentiation under chemically defined conditions. This approach allowed rapid and standardized manufacturing of billions of hiPSCs that can be produced in parallel from up to 90 different patient- and disease-specific cell lines. Moreover, we established automated multi-lineage differentiation and generated functional neurons, cardiomyocytes, and hepatocytes. To validate our approach, we compared robotic a

SUBMITTER: Tristan CA 

PROVIDER: S-EPMC8693769 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature