Synthetic nanobody-functionalized nanoparticles for accelerated development of rapid, accessible detection of viral antigens.

ABSTRACT: Successful control of emerging infectious diseases requires accelerated development of fast, affordable, and accessible assays for wide implementation at a high frequency. This paper presents a design for an in-solution assay pipeline, featuring nanobody-functionalized nanoparticles for rapid, electronic detection (Nano2RED) of Ebola and COVID-19 antigens. Synthetic nanobody binders with high affinity, specificity, and stability are selected from a combinatorial library and site-specifically conjugated to gold nanoparticles (AuNPs). Without requiring any fluorescent labelling, washing, or enzymatic amplification, these multivalent AuNP sensors reliably transduce antigen binding signals upon mixing into physical AuNP aggregation and sedimentation processes, displaying antigen-dependent opti

SUBMITTER: Chen X 

PROVIDER: S-EPMC8734080 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature