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A dual responsive fluorescent probe for selective detection of cysteine and bisulfite and its application in bioimaging.

ABSTRACT: A coumarin-based dual responsive fluorescent probe with a simple structure was developed for the detection of Cys and HSO₃ ^-. Under simulated physiological conditions, Cou-F displayed an on-off fluorescence response to Cys at 521 nm and an off-on fluorescence response to HSO₃ ^- at 500 nm. Furthermore, Cou-F had the advantages of high sensitivity, strong specificity and rapid response. The detection limits of Cou-F toward Cys and HSO₃ ^- were 0.54 μM and 0.65 μM, respectively. Cou-F enabled high selective responses to Cys and HSO₃ ^- over other biologically related species. The response times of Cou-F toward Cys and HSO₃ ^- were 80 s and 100 s. The fluorescence imaging of Cys and HSO₃ ^- was achieved in living RAW246.7 cells.

SUBMITTER: Wang X

PROVIDER: S-EPMC8978911 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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A dual responsive fluorescent probe for selective detection of cysteine and bisulfite and its application in bioimaging.

Wang Xiaofeng X Li Mingshun M Duan Tingting T Zou Yuxia Y Zhou Xuejun X

RSC advances 20211201 2

A coumarin-based dual responsive fluorescent probe with a simple structure was developed for the detection of Cys and HSO3 -. Under simulated physiological conditions, Cou-F displayed an on-off fluorescence response to Cys at 521 nm and an off-on fluorescence response to HSO3 - at 500 nm. Furthermore, Cou-F had the advantages of high sensitivity, strong specificity and rapid response. The detection limits of Cou-F toward Cys and HSO3 -< ...[more]

PMID: 35425127

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