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A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application.

ABSTRACT: A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection limit of 47.7 nM. In addition, the probe could be applied for cell imaging with low cytotoxicity.

SUBMITTER: Zeng RF

PROVIDER: S-EPMC6151380 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application.

Zeng Rui-Feng RF Lan Jin-Shuai JS Li Xiao-Die XD Liang Hui-Fen HF Liao Yan Y Lu Ying-Jie YJ Zhang Tong T Ding Yue Y

Molecules (Basel, Switzerland) 20170926 10

A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection l ...[more]

PMID: 28954423

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