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Injectable alendronate-functionalized GelMA hydrogels for mineralization and osteogenesis.

ABSTRACT: In a minimally invasive procedure, hydrogels can be injected into an affected area for drug loading and tissue repair. Here, gelatin methacryloyl (GelMA) was modified with alendronate (ALN), and three different alendronate-functionalized GelMA (GelMA-ALN) hydrogels were prepared. The modification greatly improved the swelling ratio, protein adsorption and mineralization. The GelMA-ALN hydrogels significantly promoted the in vitro osteogenic differentiation of hFOB cells as indicated by their higher alkaline phosphatase (ALP) activity, denser mineralization and up-regulated osteogenesis-related genes at both the mRNA and protein levels. Meanwhile, the cells maintained their activity and differentiated into osteoblasts when encapsulated in the GelMA-ALN hydrogels. Alendronate-modified hydrogels have potential for use in the minimally invasive treatment of irregular bone defects.

SUBMITTER: Liu L

PROVIDER: S-EPMC9081581 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Injectable alendronate-functionalized GelMA hydrogels for mineralization and osteogenesis.

Liu Lei L Li Xiaoyu X Shi Xuetao X Wang Yingjun Y

RSC advances 20180620 40

In a minimally invasive procedure, hydrogels can be injected into an affected area for drug loading and tissue repair. Here, gelatin methacryloyl (GelMA) was modified with alendronate (ALN), and three different alendronate-functionalized GelMA (GelMA-ALN) hydrogels were prepared. The modification greatly improved the swelling ratio, protein adsorption and mineralization. The GelMA-ALN hydrogels significantly promoted the <i>in vitro</i> osteogenic differentiation of hFOB cells as indicated by th ...[more]

PMID: 35539745

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