Browse
Submit Data
Databases
API
Help

Dataset Information

22 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A curcumin-based AIEE-active fluorescent probe for Cu²⁺ detection in aqueous solution.

ABSTRACT: Curcuminoids have been extensively investigated as metal ion probes, but the intrinsic aggregation-caused-quenching (ACQ) characteristic of curcumin would hinder their applications in aqueous solution. Fortunately, tetraphenylethylene (TPE) could endow the compounds with aggregation-induced emission (AIE)/aggregation-induced enhanced emission (AIEE) characteristics to eliminate the ACQ effect. According to this strategy, a series of TPE-modified curcumin derivatives L1-4 were prepared and studied for their AIEE properties. Among the four TPE-curcumin analogues, only L1 particles have been successfully used as an on-off fluorescence probe for detecting Cu²⁺ in aqueous solution. The fluorescence titration experiment determined its detection limit of 1.49 × 10^-7 mol L^-1, and the binding ratio between L1 and Cu²⁺ was estimated as 2 : 1, which was in agreement with the results of high resolution mass spectrum and Job's plot. In addition, the binding constant was evaluated as 6.77 × 10² M^-1 using a Benesi-Hildebrand plot. Finally, the obtained L1-based indicator paper showed significant fluorescence response to Cu²⁺ aqueous solution. This TPE-modified strategy improves the detection capability of curcumin probe in aqueous solution and provides a feasible way to obtain other probes with ACQ characteristics.

SUBMITTER: Lin Y

PROVIDER: S-EPMC9170379 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

A Coumarin-Based Fluorescent Probe for Ratiometric Detection of Cu2+ and Its Application in Bioimaging.

Project description:The fluorescent probe L, based on naphthalimide-modified coumarin, was designed, synthesized, and characterized, which could recognize Cu2+ from other cations selectively and sensitively in HEPES buffer (10 mM, Ph = 7. 4)/CH3CN (1:4, V/V). When the probe L interacted with Cu2+, the color and the fluorescent intensity changed obviously and it provided the naked-eye detection for Cu2+. The recognition mode between them was achieved by Job's plot, IR, MS, SEM, and 1HNMR. In addition, test strips made from L could still interact with Cu2+ in tap water effectively. The limit of detection (LOD) of L was 3.5 × 10-6 M. Additionally, the density functional theory (DFT) calculation method was used to analyze the action mechanism of L toward Cu2+. Importantly, the fluorescent probe L could demonstrate favorable selectivity toward Cu2+ in Caenorhabditis elegans. Thus, L was considered to have some potential for application in bioimaging.

| S-EPMC7573568 | biostudies-literature

A Phenanthroline-Based Fluorescent Probe for Highly Selective Detection of Extreme Alkalinity (pH?>?14) in Aqueous Solution.

Project description:Although numerous fluorescent probes are designed to detect the pH value in the past decades, developing fluorescent probes for extreme alkalinity (pH?>?14) detection in aqueous solution is still a great challenge. In this work, we utilized 1H-imidazo[4,5-f][1, 10] phenanthroline (IP) group as the recognition group of hydroxyl ion and introduced two triethylene glycol monomethyl ether groups to improve its solubility. This IP derivative, BMIP, possessed good solubility (25?mg/mL) in water. It displayed high selectivity toward extreme alkalinity (pH?>?14) over other ions and pH (from extreme acidity to pH?=?14). From 3 to 6?mol/L OH-, the exact concentration of OH- could be revealed by BMIP and the whole detection process just needed a short time (??10?s). Meanwhile, it exhibited good anti-interference ability and repeatability during the detection process. Through optical spectra and NMR analysis, its detection mechanism was proved to be deprotonation by hydroxyl ion and then aggregation-induced enhanced emission. Our study presents a new basic group based on which researchers can develop new fluorescent probes that can detect extreme alkalinity (pH?>?14) in aqueous solution.

| S-EPMC6751243 | biostudies-literature

Novel "Turn-On" Fluorescent Probe for Highly Selectively Sensing Fluoride in Aqueous Solution Based on Tb3+-Functionalized Metal-Organic Frameworks.

Project description:A Zr-based metal-organic framework (Zr-MOF) which has free carbonyl groups is synthesized successfully through mix-ligand strategy. Subsequently, Tb3+ is encapsulated into a Zr-MOF by postcoordinated modification. The Tb3+@Zr-MOF exhibits the characteristic emission of Tb3+ because of efficient sensitization through antenna effects. The Tb3+@Zr-MOF is further developed as a novel "turn-on" fluorescent probe to detect fluoride ions in aqueous solution. The results show that Tb3+@Zr-MOF exhibits excellent selectivity, high stability, low detection limits, and good anti-interference for sensitizing fluoride ions. In addition, the possible sensing mechanism that the induced luminescence properties may be attributed to Lewis acid-base interactions is discussed.

| S-EPMC6645131 | biostudies-literature

A fluorescent probe for rapid aqueous fluoride detection and cell imaging.

Project description:A stable and highly selective fluorescent probe has been designed and synthesized for the rapid detection of fluoride ions (F(-)) in aqueous solution and living cells. The design was based on the high reactivity of F(-) toward a silyl group.

| S-EPMC3587330 | biostudies-literature

A New Fluorescent Probe for Hydrogen Sulfide Detection in Solution and Living Cells.

Project description:Since Hydrogen Sulfide (H2S) was recognized as a gas transmitter, its detection and quantification have become a hot research topic among chemists and biologists. In this area, fluorescent probes have shown great advantages: fast and strong response, low detection limit and easy manipulation. Here we developed a new fluorescent probe that detected H2S selectively among various bioactive and inorganic salts. This probe was based on the core structure of fluorescein and reacted with H2S through azide-reduction. Great linearity was achieved correlating fluorescence intensity and H2S concentrations in solution. The detection of H2S in cancer cells was also achieved.

| S-EPMC10488361 | biostudies-literature

Detection of Active Granzyme A in NK92 Cells with Fluorescent Activity-Based Probe.

Project description:Cytotoxic T-lymphocytes (CTLs) and natural killer cells (NKs) kill compromised cells to defend against tumor and viral infections. Both effector cell types use multiple strategies to induce target cell death including Fas/CD95 activation and the release of perforin and a group of lymphocyte granule serine proteases called granzymes. Granzymes have relatively broad and overlapping substrate specificities and may hydrolyze a wide range of peptidic epitopes; it is therefore challenging to identify their natural and synthetic substrates and to distinguish their localization and functions. Here, we present a specific and potent substrate, an inhibitor, and an activity-based probe of Granzyme A (GrA) that can be used to follow functional GrA in cells.

| S-EPMC7590976 | biostudies-literature

A Fast-Response AIE-Active Ratiometric Fluorescent Probe for the Detection of Carboxylesterase.

Project description:Hepatocellular carcinoma (HCC) is associated with a high mortality rate worldwide. The therapeutic outcomes can be significantly improved if diagnosis and treatment are initiated earlier in the disease process. Recently, the carboxylesterase (CaE) activity/level in human plasma was reported to be a novel serological biomarker candidate for HCC. In this article, we fabricated a new fluorescent probe with AIE characteristics for the rapid detection of CaE with a more reliable ratiometric response mode. The TCFISE probe showed high sensitivity (LOD: 93.0 μU/mL) and selectivity toward CaE. Furthermore, the good pH stability, superior resistance against photobleaching, and low cytotoxicity highlight the high potential of the TCFISE probe for application in the monitoring of CaE activity in complex biological samples and in live cells, tissues, and animals.

| S-EPMC9313056 | biostudies-literature

Coumarin-Based Dual Chemosensor for Colorimetric and Fluorescent Detection of Cu2+ in Water Media.

Project description:A novel coumarin derivative (5) was synthesized and used as a colorimetric and fluorescent probe for selective detection of Cu2+ ions in the presence of other metal ions, with the detection limits of 5.7 and 4.0 ppb, respectively. Cu2+ ion reacts with probe 5 to form a 1:1 stoichiometry complex, resulting in a remarkable redshift of absorption maximum from 460 to 510 nm, as well as almost completely quenching fluorescence intensity of probe 5 at the wavelength of 536 nm. These changes can be distinctly observed by naked eyes. In addition, the working pH range of probe 5 is wide and suitable for physiological conditions, thus probe 5 may be used for detection of Cu2+ ions in living cells. The stable structures of probe 5 and its 1:1 complex with Cu2+ ion were optimized at the PBE0/6-31+G(d) level of theory. The presence and characteristics of bonds in compounds were studied through atoms in a molecule and natural bond orbital analysis. The formation of the complex led to a strong transfer of electron density from probe 5 as a ligand to Cu2+ ion, resulting in breaking the π-electron conjugated system, which is the cause of fluorescence quenching and color change of 5-Cu2+ complex.

| S-EPMC7450636 | biostudies-literature

Sensing Zn2+ in Aqueous Solution with a Fluorescent Scorpiand Macrocyclic Ligand Decorated with an Anthracene Bearing Tail.

Project description:Synthesis of the new scorpiand ligand L composed of a [9]aneN3 macrocyclic ring bearing a CH2CH2NHCH2-anthracene tail is reported. L forms both cation (Zn2+) and anion (phosphate, benzoate) complexes. In addition, the zinc complexes of L bind these anions. The equilibrium constants for ligand protonation and complex formation were determined in 0.1 M NaCl aqueous solution at 298.1 ± 0.1 K by means of potentiometric (pH-metric) titrations. pH Controlled coordination/detachment of the ligand tail to Zn2+ switch on and off the fluorescence emission from the anthracene fluorophore. Accordingly, L is able to sense Zn2+ in the pH range 6-10 down to nM concentrations of the metal ion. L can efficiently sense Zn2+ even in the presence of large excess of coordinating anions, such as cyanide, sulphide, phosphate and benzoate, despite their ability to bind the metal ion.

| S-EPMC7146481 | biostudies-literature

Pyrene-Based "Turn-Off" Probe with Broad Detection Range for Cu2+ , Pb2+ and Hg2+ Ions.

Project description:Detection of metals in different environments with high selectivity and specificity is one of the prerequisites of the fight against environmental pollution with these elements. Pyrenes are well suited for the fluorescence sensing in different media. The applied sensing principle typically relies on the formation of intra- and intermolecular excimers, which is however limiting the sensitivity range due to masking of e. g. quenching effects by the excimer emission. Herein we report a highly selective, structurally rigid chemical sensor based on the monomer fluorescence of pyrene moieties bearing triazole groups. This sensor can quantitatively detect Cu2+ , Pb2+ and Hg2+ in organic solvents over a broad concentrations range, even in the presence of ubiquitous ions such as Na+ , K+ , Ca2+ and Mg2+ . The strongly emissive sensor's fluorescence with a long lifetime of 165 ns is quenched by a 1 : 1 complex formation upon addition of metal ions in acetonitrile. Upon addition of a tenfold excess of the metal ion to the sensor, agglomerates with a diameter of about 3 nm are formed. Due to complex interactions in the system, conventional linear correlations are not observed for all concentrations. Therefore, a critical comparison between the conventional Job plot interpretation, the method of Benesi-Hildebrand, and a non-linear fit is presented. The reported system enables the specific and robust sensing of medically and environmentally relevant ions in the health-relevant nM range and could be used e. g. for the monitoring of the respective ions in waste streams.

| S-EPMC8251986 | biostudies-literature