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An ICT-Based Coumarin Fluorescent Probe for the Detection of Hydrazine and Its Application in Environmental Water Samples and Organisms.

ABSTRACT: As an inorganic small molecule pollutant, the toxicity and potential carcinogenicity of hydrazine (N₂H₄) are of increasing concern. In this work, A water-soluble fluorescent probe (OCYB) based on the intramolecular charge transfer (ICT) mechanism for the detection of hydrazine was designed and synthesized. Taking the advantage of 4-bromobutyryl as the recognition group, the high selectivity of OCYB to N₂H₄ was confirmed by steady-state fluorescence spectroscopy. The limit of detection (LOD) was calculated to be 78 nM in the DMSO-HEPES (pH 7.4) system. The detection mechanism was verified by NMR, HRMS and density functional theory (DFT) calculations. In addition, OCYB exhibits strong anti-interference ability and an "Off-On" fluorescence enhancement effect. Importantly, OCYB can be used to effectively monitor the fluorescence distribution of N₂H₄ in environmental water samples and organisms.

SUBMITTER: Liu X

PROVIDER: S-EPMC9237640 | biostudies-literature |

REPOSITORIES: biostudies-literature

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