Ontology highlight
ABSTRACT:
SUBMITTER: Krell K
PROVIDER: S-EPMC9297951 | biostudies-literature | 2021 Nov
REPOSITORIES: biostudies-literature
Chemistry (Weinheim an der Bergstrasse, Germany) 20211029 65
Sydnones are highly stable mesoionic 1,3-dipoles that react with cyclooctynes through strain-promoted sydnone-alkyne cycloaddition (SPSAC). Although sydnones have been shown to be valuable bioorthogonal chemical reporters for the labeling of proteins and complex glycans, nucleic acids have not yet been tagged by SPSAC. Evaluation of SPSAC kinetics with model substrates showed fast reactions with cyclooctyne probes (up to k=0.59 M<sup>-1</sup> s<sup>-1</sup> ), and two different sydnones were ef ...[more]