Browse
Submit Data
Databases
API
Help

Dataset Information

16 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors.

ABSTRACT: During product isolation the received bioreceptors often do not exhibit a sufficient biochemical activity due to multistep dissociation and loss of cofactors. However, for bioelectrochemical applications the presence of cofactors is necessary for a successful oxidative or reductive conversion of the substrates to the products. Herein, we show how the immobilization of the required electroplated cofactors in a design of amperometric electrodes can in situ assist the activity of apo-enzymes. Compared to conventional approaches used in enzyme engineering this tailored nanoengineering methodology is superior from economic point of view, labor and time costs, storage conditions, reduced amount of waste and can fill the gap in the development of tuned bioelectrocatalysts.

SUBMITTER: Apushkinskaya N

PROVIDER: S-EPMC9307585 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

The CoFactor database: organic cofactors in enzyme catalysis.

Project description:MotivationOrganic enzyme cofactors are involved in many enzyme reactions. Therefore, the analysis of cofactors is crucial to gain a better understanding of enzyme catalysis. To aid this, we have created the CoFactor database.ResultsCoFactor provides a web interface to access hand-curated data extracted from the literature on organic enzyme cofactors in biocatalysis, as well as automatically collected information. CoFactor includes information on the conformational and solvent accessibility variation of the enzyme-bound cofactors, as well as mechanistic and structural information about the hosting enzymes.AvailabilityThe database is publicly available and can be accessed at http://www.ebi.ac.uk/thornton-srv/databases/CoFactor.

| S-EPMC2944199 | biostudies-literature

Detecting and utilizing minority phases in heterogeneous catalysis.

Project description:Highly active phases in carbon monoxide oxidation are known, however they are transient in nature. Here, we determined for the first time the structure of such a highly active phase on platinum nanoparticles in an actual reactor. Unlike generally assumed, the surface of this phase is virtually free of adsorbates and co-exists with carbon-monoxide covered and surface oxidized platinum. Understanding the relation between gas composition and catalyst structure at all times and locations within a reactor enabled the rational design of a reactor concept, which maximizes the amount of the highly active phase and minimizes the amount of platinum needed.

| S-EPMC5121607 | biostudies-literature

Zinc anode-compatible in-situ solid electrolyte interphase via cation solvation modulation.

Project description:The surface chemistry of solid electrolyte interphase is one of the critical factors that govern the cycling life of rechargeable batteries. However, this chemistry is less explored for zinc anodes, owing to their relatively high redox potential and limited choices in electrolyte. Here, we report the observation of a zinc fluoride-rich organic/inorganic hybrid solid electrolyte interphase on zinc anode, based on an acetamide-Zn(TFSI)2 eutectic electrolyte. A combination of experimental and modeling investigations reveals that the presence of anion-complexing zinc species with markedly lowered decomposition energies contributes to the in situ formation of an interphase. The as-protected anode enables reversible (~100% Coulombic efficiency) and dendrite-free zinc plating/stripping even at high areal capacities (>2.5?mAh?cm?2), endowed by the fast ion migration coupled with high mechanical strength of the protective interphase. With this interphasial design the assembled zinc batteries exhibit excellent cycling stability with negligible capacity loss at both low and high rates.

| S-EPMC6879498 | biostudies-literature

Continuous proline catalysis via leaching of solid proline.

Project description:Herein, we demonstrate that a homogeneous catalyst can be prepared continuously via reaction with a packed-bed of a catalyst precursor. Specifically, we perform continuous proline catalyzed α-aminoxylations using a packed-bed of L-proline. The system relies on a multistep sequence in which an aldehyde and thiourea additive are passed through a column of solid proline, presumably forming a soluble oxazolidinone intermediate. This transports a catalytic amount of proline from the packed-bed into the reactor coil for subsequent combination with a solution of nitrosobenzene, affording the desired optically active α-aminooxy alcohol after reduction. To our knowledge, this is the first example in which a homogeneous catalyst is produced continuously using a packed-bed. We predict that the method will not only be useful for other L-proline catalyzed reactions, but we also foresee that it could be used to produce other catalytic species in flow.

| S-EPMC3252872 | biostudies-literature

Sustainable pathway to furanics from biomass via heterogeneous organo-catalysis.

Project description:An organic sulfonated graphitic carbon nitride is synthesized and its application has been demonstrated in the conversion of carbohydrates into furanics and related value-added products. The most important feature of the material is the stability and acidity, which could be utilized at elevated temperatures for cleaving carbohydrates and converting them into biologically important scaffolds and platform chemicals.

| S-EPMC6178834 | biostudies-literature

Riboswitch effectors as protein enzyme cofactors.

Project description:The recently identified glmS ribozyme revealed that RNA enzymes, like protein enzymes, are capable of using small molecules as catalytic cofactors to promote chemical reactions. Flavin mononucleotide (FMN), S-adenosyl methionine (SAM), adenosyl cobalamin (AdoCbl), and thiamine pyrophosphate (TPP) are known ligands for RNA riboswitches in the control of gene expression, but are also catalytically powerful and ubiquitous cofactors in protein enzymes. If RNA, instead of just binding these molecules, could harness the chemical potential of the cofactor, it would significantly expand the enzymatic repertoire of ribozymes. Here we review the chemistry of AdoCbl, SAM, FMN, and TPP in protein enzymology and speculate on how these cofactors might have been used by ribozymes in the prebiotic RNA World or may still find application in modern biology.

| S-EPMC2390802 | biostudies-literature

Pulsed Photothermal Heterogeneous Catalysis

Project description:Anthropogenic climate change urgently calls for the greening

| S-EPMC9990069 | biostudies-literature

Finding enzyme cofactors in Protein Data Bank.

Project description:MOTIVATION:Cofactors are essential for many enzyme reactions. The Protein Data Bank (PDB) contains >67?000 entries containing enzyme structures, many with bound cofactor or cofactor-like molecules. This work aims to identify and categorize these small molecules in the PDB and make it easier to find them. RESULTS:The Protein Data Bank in Europe (PDBe; pdbe.org) has implemented a pipeline to identify enzyme cofactor and cofactor-like molecules, which are now part of the PDBe weekly release process. AVAILABILITY AND IMPLEMENTATION:Information is made available on the individual PDBe entry pages at pdbe.org and programmatically through the PDBe REST API (pdbe.org/api). SUPPLEMENTARY INFORMATION:Supplementary data are available at Bioinformatics online.

| S-EPMC6748742 | biostudies-literature

Bifunctional MOFs in Heterogeneous Catalysis.

Project description:The ever-increasing landscape of heterogeneous catalysis, pure and applied, utilizes many different catalysts. Academic insights along with many industrial adaptations paved the way for the growth. In designing a catalyst, it is desirable to have a priori knowledge of what structure needs to be targeted to help in achieving the goal. When focusing on catalysis, one needs to cope with a vast corpus of knowledge and information. The overwhelming desire to exploit catalysis toward commercial ends is irresistible. In today's world, one of the requirements of developing a new catalyst is to address the environmental concerns. The well-established heterogeneous catalysts have microporous structures (<25 Å), which find use in many industrial processes. The metal-organic framework (MOF) compounds, being pursued vigorously during the last two decades, have similar microporosity with well-defined pores and channels. The MOFs possess large surface area and assemble to delicate structural and compositional variations either during the preparation or through postsynthetic modifications (PSMs). The MOFs, in fact, offer excellent scope as simple Lewis acidic, Brönsted acidic, Lewis basic, and more importantly bifunctional (acidic as well as basic) agents for carrying out catalysis. The many advances that happened over the years in biology helped in the design of many good biocatalysts. The tools and techniques (advanced preparative approaches coupled with computational insights), on the other hand, have helped in generating interesting and good inorganic catalysts. In this review, the recent advances in bifunctional catalysis employing MOFs are presented. In doing so, we have concentrated on the developments that happened during the past decade or so.

| S-EPMC10853920 | biostudies-literature

Redox switchable catalysis utilizing a fluorescent dye.

Project description:This report describes the implementation of a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) dye into the ligand framework of a Rh-based catalyst. The redox-active nature of the BODIPY dye is utilized to generate a catalyst that is capable of exhibiting redox-switchable catalytic behavior for the hydroboration of alkenes through a BODIPY-based reduction.

| S-EPMC6953248 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data