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Tilt series of dividing vegetative and sporulating cells of Bacillus subtilis from the manuscript - Khanna et al., 2021


ABSTRACT:

SUBMITTER: Elizabeth Villa 

PROVIDER: EMPIAR-10710 | biostudies-other |

REPOSITORIES: biostudies-other

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Asymmetric localization of the cell division machinery during <i>Bacillus subtilis</i> sporulation.

Khanna Kanika K   Lopez-Garrido Javier J   Sugie Joseph J   Pogliano Kit K   Villa Elizabeth E  

eLife 20210521


The Gram-positive bacterium <i>Bacillus subtilis</i> can divide via two modes. During vegetative growth, the division septum is formed at the midcell to produce two equal daughter cells. However, during sporulation, the division septum is formed closer to one pole to yield a smaller forespore and a larger mother cell. Using cryo-electron tomography, genetics and fluorescence microscopy, we found that the organization of the division machinery is different in the two septa. While FtsAZ filaments,  ...[more]

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