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Sub-2 Å Single-Particle Cryo-EM Reconstruction of AAV2-L336C


ABSTRACT:

SUBMITTER: Yong Zi Tan 

PROVIDER: EMPIAR-10202 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant.

Tan Yong Zi YZ   Aiyer Sriram S   Mietzsch Mario M   Hull Joshua A JA   McKenna Robert R   Grieger Joshua J   Samulski R Jude RJ   Baker Timothy S TS   Agbandje-McKenna Mavis M   Lyumkis Dmitry D  

Nature communications 20180907 1


Single-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures have lagged behind microscope capabilities. Here, we exploit several technical advances to improve resolution, including per-particle contrast transfer function (CTF) refinement and correction for Ewald sphere curvature. The latter is demonstrated with several experimental samples and should become more  ...[more]

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