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Sub-2 Å Single-Particle Cryo-EM Reconstruction of AAV2-L336C

ABSTRACT:

SUBMITTER: Yong Zi Tan

PROVIDER: EMPIAR-10202 | biostudies-other |

REPOSITORIES: biostudies-other

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Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant.

Tan Yong Zi YZ Aiyer Sriram S Mietzsch Mario M Hull Joshua A JA McKenna Robert R Grieger Joshua J Samulski R Jude RJ Baker Timothy S TS Agbandje-McKenna Mavis M Lyumkis Dmitry D

Nature communications 20180907 1

Single-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures have lagged behind microscope capabilities. Here, we exploit several technical advances to improve resolution, including per-particle contrast transfer function (CTF) refinement and correction for Ewald sphere curvature. The latter is demonstrated with several experimental samples and should become more ...[more]

PMID: 30194371

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