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Movies of apoferritin collected at different dose rates on the Direct Electron Apollo direct detector - 60 eps

ABSTRACT:

SUBMITTER: Scott Stagg 

PROVIDER: EMPIAR-11261 | biostudies-other |

REPOSITORIES: biostudies-other

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Characterizing the resolution and throughput of the Apollo direct electron detector.

Peng Ruizhi R   Fu Xiaofeng X   Mendez Joshua H JH   Randolph Peter S PS   Bammes Benjamin E BE   Stagg Scott M SM  

Journal of structural biology: X 20221205

Advances in electron detection have been essential to the success of high-resolution cryo-EM structure determination. A new generation of direct electron detector called the Apollo, has been developed by Direct Electron. The Apollo uses a novel event-based MAPS detector custom designed for ultra-fast electron counting. We have evaluated this new camera, finding that it delivers high detective quantum efficiency (DQE) and low coincidence loss, enabling high-quality electron counting data acquisit  ...[more]

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