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Single particle cryo EM dataset of SARS-CoV-2 Spike protein in uncleavable form, which generated a 2.6Å reconstruction of the closed trimer.

ABSTRACT:

SUBMITTER: Donald Benton

PROVIDER: EMPIAR-10469 | biostudies-other |

REPOSITORIES: biostudies-other

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SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects.

Wrobel Antoni G AG Benton Donald J DJ Xu Pengqi P Roustan Chloë C Martin Stephen R SR Rosenthal Peter B PB Skehel John J JJ Gamblin Steven J SJ

Nature structural & molecular biology 20200709 8

SARS-CoV-2 is thought to have emerged from bats, possibly via a secondary host. Here, we investigate the relationship of spike (S) glycoprotein from SARS-CoV-2 with the S protein of a closely related bat virus, RaTG13. We determined cryo-EM structures for RaTG13 S and for both furin-cleaved and uncleaved SARS-CoV-2 S; we compared these with recently reported structures for uncleaved SARS-CoV-2 S. We also biochemically characterized their relative stabilities and affinities for the SARS-CoV-2 rec ...[more]

PMID: 32647346

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